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Literature summary for 1.10.3.2 extracted from
- Purification and characterization of laccase from the white rot fungus Trametes versicolor (2005), J. Microbiol., 43, 555-560.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0128 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 50°C | Trametes versicolor |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Trametes versicolor | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Trametes versicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97000 | - |
1 * 97000, SDS-PAGE | Trametes versicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes versicolor | - |
isolated in Korea | - |
| Trametes versicolor 951022 | - |
isolated in Korea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 209fold from strain 951022 by ethanol precipitation, anion exchange and hydrophobic interaction chromatography, and gel filtration, to homogeneity | Trametes versicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 91440 | - |
purified enzyme | Trametes versicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes versicolor | ? | - |
? | |
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes versicolor 951022 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 97000, SDS-PAGE | Trametes versicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Trametes versicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
50 | purified enzyme, stable | Trametes versicolor |
| 60 | - |
1 h, loss of 40% activity, purified enzyme | Trametes versicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Trametes versicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 4 | purified enzyme, stable | Trametes versicolor |
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