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Literature summary for 1.10.3.2 extracted from

  • Casella, L.; Gullotti, M.; Monzani, E.; Santagostini, L.; Zoppellaro, G.; Sakurai, T.
    Enzymatic and spectroscopic studies on the activation or inhibition effects by substituted pHenolic compounds in the oxidation of aryldiamines and catechols catalyzed by Rhus vernicifera laccase (2006), J. Inorg. Biochem., 100, 2127-2139.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-chlorophenol activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
2-Fluorophenol activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
4-Chlorophenol activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
4-Fluorophenol activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxyphenylacetic acid effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
4-hydroxyphenylpropionic acid effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
4-nitrophenol effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics in absence and presence of acidic and halophenols, overview Toxicodendron vernicifluum
2.3 2.5 N,N-dimethyl-4-phenylenediamine pH 7.0, 20°C Toxicodendron vernicifluum
3.3 3.8 N,N-dimethyl-4-phenylenediamine pH 7.0, 25°C Toxicodendron vernicifluum

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ copper-containing enzyme, trinuclear Cu cluster upon interaction with o-fluorophenol, overview Toxicodendron vernicifluum

Organism

Organism UniProt Comment Textmining
Toxicodendron vernicifluum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-tert-butylcatechol + O2
-
Toxicodendron vernicifluum ?
-
?
N,N-dimethyl-4-phenylenediamine + O2
-
Toxicodendron vernicifluum ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Toxicodendron vernicifluum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
181 186 N,N-dimethyl-4-phenylenediamine pH 7.0, 20°C Toxicodendron vernicifluum
350 443 N,N-dimethyl-4-phenylenediamine pH 7.0, 25°C Toxicodendron vernicifluum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Toxicodendron vernicifluum

pH Range

pH Minimum pH Maximum Comment Organism
4.3 9
-
Toxicodendron vernicifluum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics with acidic phenols, overview Toxicodendron vernicifluum