General Stability | Organism |
---|---|
ammonium sulfate stabilizes | Sinorhizobium meliloti |
no satnilization by EDTA and Cu2+ | Sinorhizobium meliloti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Sinorhizobium meliloti | |
Cu2+ | - |
Sinorhizobium meliloti | |
Fe3+ | - |
Sinorhizobium meliloti | |
Mn2+ | - |
Sinorhizobium meliloti | |
NaCl | - |
Sinorhizobium meliloti | |
NaCN | - |
Sinorhizobium meliloti | |
NaF | - |
Sinorhizobium meliloti | |
reduced glutathione | - |
Sinorhizobium meliloti |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Sinorhizobium meliloti | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 4fold activation | Sinorhizobium meliloti | |
imidazole | about 2fold activation | Sinorhizobium meliloti | |
K2SO4 | 3.2fold activation | Sinorhizobium meliloti | |
Na2SO4 | 3.5fold activation | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
- |
- |
Sinorhizobium meliloti CE52G | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 29fold by a two-step procedure involving heat treatment and immobilized metal affinity chromatography | Sinorhizobium meliloti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.402 | - |
purified enzyme | Sinorhizobium meliloti |
Storage Stability | Organism |
---|---|
the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4 | Sinorhizobium meliloti |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Sinorhizobium meliloti |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4 | Sinorhizobium meliloti |
75 | - |
purified enzyme, inactivation | Sinorhizobium meliloti |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4 | Sinorhizobium meliloti |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sinorhizobium meliloti | isoelectric focusing | - |
6.2 |