Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the extracellular laccase production is enhanced up to 2.5-fold in the medium amended with xylidine at 1 mM | Lentinus sajor-caju |
Application | Comment | Organism |
---|---|---|
additional information | the enzyme is useful in decolorization of azo dyes, method optimization, overview | Lentinus sajor-caju |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaN3 | complete inhibition | Lentinus sajor-caju |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Lentinus sajor-caju | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | copper-containing enzyme, type 1 and type 3 copper | Lentinus sajor-caju |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
gel filtration and native PAGE | Lentinus sajor-caju |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentinus sajor-caju | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 10.3fold by ammonium sulfate fractionation, anion exchange chromatography and gel filtration | Lentinus sajor-caju |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | submerged culture | Lentinus sajor-caju | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Lentinus sajor-caju | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Lentinus sajor-caju | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | - |
? | |
4-phenylenediamine + O2 | - |
Lentinus sajor-caju | ? | - |
? | |
catechol + O2 | - |
Lentinus sajor-caju | ? | - |
? | |
guaiacol + O2 | - |
Lentinus sajor-caju | ? | - |
? | |
additional information | substrate specificity in descending order: syringaldazine, 4-phenylenediamine, 2,6-dimethoxyphenol, guaiacol, catechol, the purified enzyme decolorizes azo dyes, such as acid red 18, acid Black 1, and direct blue 71, up to 90, 87, and 72%, respectively, optimization, overview | Lentinus sajor-caju | ? | - |
? | |
syringaldazine + O2 | - |
Lentinus sajor-caju | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 61000, SDS-PAGE | Lentinus sajor-caju |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lentinus sajor-caju |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | sharp decrease in activity above 40°C | Lentinus sajor-caju |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified enzyme, highly stable at | Lentinus sajor-caju |
50 | - |
purified enzyme, unstable above | Lentinus sajor-caju |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | - |
Lentinus sajor-caju |