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Literature summary for 1.10.3.2 extracted from
- Laccase from the medicinal mushroom Agaricus blazei: production, purification and characterization (2005), Appl. Microbiol. Biotechnol., 67, 357-363.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | 90% inhibition at 0.3 M, completely reversible | Agaricus blazei |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
syringaldazine | pH 5.5 | Agaricus blazei | |
| 0.063 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.5 | Agaricus blazei | |
| 1.026 | - |
2,6-dimethoxyphenol | pH 5.5 | Agaricus blazei | |
| 4.31 | - |
guaiacol | pH 5.5 | Agaricus blazei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
gel filtration | Agaricus blazei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agaricus blazei | - |
medicinal mushroom | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 8.4fold by ultrafiltration, two steps of anion and one of cation exchange chromatography | Agaricus blazei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Agaricus blazei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Agaricus blazei | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Agaricus blazei | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | - |
? | |
| guaiacol + O2 | - |
Agaricus blazei | ? | - |
? | |
| syringaldazine + O2 | - |
Agaricus blazei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, SDS-PAGE | Agaricus blazei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
purified laccase is stable at 20°C, pH 7.0 and pH 3.0 | Agaricus blazei |
| 40 | - |
purified laccase rapidly loses its activity at 40°C | Agaricus blazei |
| 60 | - |
10 h, almost complete inactivation | Agaricus blazei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
syringaldazine | pH 5.5 | Agaricus blazei | |
| 15 | - |
2,6-dimethoxyphenol | pH 5.5 | Agaricus blazei | |
| 21 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.5 | Agaricus blazei | |
| 159 | - |
guaiacol | pH 5.5 | Agaricus blazei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
substrate-dependent | Agaricus blazei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | purified laccase is stable at 20°C, pH 7.0 and pH 3.0 | Agaricus blazei |
| 10 | - |
purified laccase rapidly loses its activity at pH 10 | Agaricus blazei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Agaricus blazei | isoelectric focusing | - |
4 |
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