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Literature summary for 1.10.3.2 extracted from

  • Pozdnyakova, N.N.; Turkovskaya, O.V.; Yudina, E.N.; Rodakiewicz-Nowak, Y.
    Yellow laccase from the fungus Pleurotus ostreatus D1: purification and characterization (2006), Appl. Biochem. Microbiol., 42, 56-61.
No PubMed abstract available
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol complete inhibition at 0.1 mM Pleurotus ostreatus
EDTA 60% inhibition at 100 mM Pleurotus ostreatus
SDS complete inhibition at 140 mM Pleurotus ostreatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
 syringaldazine pH 7.0, 25°C Pleurotus ostreatus
0.11
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, 25°C Pleurotus ostreatus
0.43
-
 2,6-dimethoxyphenol pH 7.0, 25°C Pleurotus ostreatus
3.65
-
 o-benzenediol pH 7.0, 25°C Pleurotus ostreatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pleurotus ostreatus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ copper-containing enzyme, type I copper atoms Pleurotus ostreatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 1 * 64000, SDS-PAGE Pleurotus ostreatus
66000
-
 gel filtration Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
-
-
Pleurotus ostreatus D1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration Pleurotus ostreatus

Source Tissue

Source Tissue Comment Organism Textmining
additional information solid-phase culture, natural substrate are sunflower-seed shells Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pleurotus ostreatus
5.8
-
 purified enzyme, substrate syringaldazine Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Pleurotus ostreatus ?
-
 ?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Pleurotus ostreatus D1 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Pleurotus ostreatus 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
2,6-dimethoxyphenol + O2
-
 Pleurotus ostreatus D1 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
additional information no activity with tyrosine and hydroquinone, the enzyme in absence of a mediator oxidizes anthracene to anthraquinone to 95% Pleurotus ostreatus ?
-
 ?
additional information no activity with tyrosine and hydroquinone, the enzyme in absence of a mediator oxidizes anthracene to anthraquinone to 95% Pleurotus ostreatus D1 ?
-
 ?
pyrocatechol + O2
-
 Pleurotus ostreatus ?
-
 ?
pyrocatechol + O2
-
 Pleurotus ostreatus D1 ?
-
 ?
syringaldazine + O2
-
 Pleurotus ostreatus ?
-
 ?
syringaldazine + O2
-
 Pleurotus ostreatus D1 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 64000, SDS-PAGE Pleurotus ostreatus

Synonyms

Synonyms Comment Organism
yellow laccase
-
 Pleurotus ostreatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pleurotus ostreatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04 1.97 2,6-dimethoxyphenol pH 7.0, 25°C Pleurotus ostreatus
2 8 o-benzenediol pH 7.0, 25°C Pleurotus ostreatus
6.1
-
 syringaldazine pH 7.0, 25°C Pleurotus ostreatus
8.94
-
 2,6-dimethoxyphenol pH 7.0, 25°C Pleurotus ostreatus
11.7
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, 25°C Pleurotus ostreatus
21.98
-
 o-benzenediol pH 7.0, 25°C Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 8 dependent on the substrate, overview Pleurotus ostreatus

pH Range

pH Minimum pH Maximum Comment Organism
2 8.8
-
 Pleurotus ostreatus