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Literature summary for 1.10.3.2 extracted from
- Affinity chromatography: a convenient method for purification of fungal lactases (1991), Biotechnol. Bioeng., 37, 770-777.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | comparison of values for free and immobilized laccase | Fomes fomentarius | |
| 0.03 | - |
syringic acid | immobilized enzyme | Fomes fomentarius |  |
| 0.055 | - |
syringic acid | immobilized enzyme | Fomes fomentarius | |
| 0.06 | - |
vanillic acid | immobilized enzyme | Trametes versicolor | |
| 0.13 | - |
syringic acid | - |
Trametes versicolor | |
| 0.28 | - |
vanillic acid | - |
Trametes versicolor | |
| 0.28 | - |
syringic acid | - |
Fomes fomentarius | |
| 0.97 | - |
vanillic acid | immobilized enzyme | Fomes fomentarius | |
| 2.92 | - |
vanillic acid | - |
Fomes fomentarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trametes versicolor | - |
- |
| extracellular | - |
Fomes fomentarius | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fomes fomentarius | - |
- |
- |
| Trametes versicolor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trametes versicolor |
- |
Fomes fomentarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Trametes versicolor | - |
| culture filtrate | - |
Fomes fomentarius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
depending on isozyme, substrate and pH | Trametes versicolor |
| additional information | - |
depending on isozyme, substrate and pH | Fomes fomentarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 syringic acid + O2 | - |
Trametes versicolor | 4 4-carboxy-2,6-dimethoxyphenoxyl + 2 H2O | - |
? | |
| 4 syringic acid + O2 | - |
Fomes fomentarius | 4 4-carboxy-2,6-dimethoxyphenoxyl + 2 H2O | - |
? | |
| ferulic acid + O2 | - |
Trametes versicolor | 3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O | - |
? | |
| ferulic acid + O2 | - |
Fomes fomentarius | 3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O | - |
? | |
| sinapic acid + O2 | - |
Trametes versicolor | 3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O | - |
? | |
| sinapic acid + O2 | - |
Fomes fomentarius | 3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O | - |
? | |
| vanillic acid + O2 | - |
Trametes versicolor | 4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O | - |
? | |
| vanillic acid + O2 | - |
Fomes fomentarius | 4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
immobilized | Fomes fomentarius |
| 52 | - |
free enzyme | Fomes fomentarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
dependency on isozyme, substrate and organism | Trametes versicolor |
| additional information | - |
dependency on isozyme, substrate and organism | Fomes fomentarius |
| 3.5 | - |
substrate sinapic acid | Trametes versicolor |
| 3.8 | - |
substrate sinapic acid | Fomes fomentarius |
| 3.8 | 4.8 | depending on substrate and organism | Trametes versicolor |
| 3.8 | 4.8 | depending on substrate and organism | Fomes fomentarius |
| 4 | - |
substrate ferulic acid | Trametes versicolor |
| 4.8 | - |
substrate ferulic acid | Fomes fomentarius |
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