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Literature summary for 1.10.3.11 extracted from

  • Kido, Y.; Shiba, T.; Inaoka, D.K.; Sakamoto, K.; Nara, T.; Aoki, T.; Honma, T.; Tanaka, A.; Inoue, M.; Matsuoka, S.; Moore, A.; Harada, S.; Kita, K.
    Crystallization and preliminary crystallographic analysis of cyanide-insensitive alternative oxidase from Trypanosoma brucei brucei (2010), Acta Crystallogr. Sect. F, 66, 275-278.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.10.3.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in heme-deficient Escherichia coli BL21(DE3) cells Trypanosoma brucei brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 28-34% (w/v) PEG 400, 100 mM imidazole buffer pH 7.4, 500 mM potassium formate and 0.4% (w/v) C8E4l Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
ascofuranone specifically inhibits the quinol oxidase activity of TAO Trypanosoma brucei brucei
additional information insensitive to cyanide Trypanosoma brucei brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane
-
 Trypanosoma brucei brucei 31966
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Trypanosoma brucei brucei
110000
-
 gel filtration Trypanosoma brucei brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei
-
-
-
Trypanosoma brucei brucei FN102
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultracentrifugation and Talon Co2+-affinity column chromatography Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquinol + O2 a non-proton-pumping ubiquinol oxidase that catalyzes the fourelectron reduction of dioxygen to water Trypanosoma brucei brucei ubiquinone + H2O
-
 ?
ubiquinol + O2 a non-proton-pumping ubiquinol oxidase that catalyzes the fourelectron reduction of dioxygen to water Trypanosoma brucei brucei FN102 ubiquinone + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, SDS-PAGE Trypanosoma brucei brucei

Synonyms

Synonyms Comment Organism
AOX
-
 Trypanosoma brucei brucei
cyanide-insensitive alternative oxidase
-
 Trypanosoma brucei brucei
TAO
-
 Trypanosoma brucei brucei