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Literature summary for 1.10.3.1 extracted from

  • Cheema, S.; Sommerhalter, M.
    Characterization of polyphenol oxidase activity in Ataulfo mango (2015), Food Chem., 171, 382-387 .
    View publication on PubMed
Search for more literature for 1.10.3.1

Activating Compound

Activating Compound Comment Organism Structure
SDS enhances the PPO activity in fruits, with pulp showing a stronger increase than skin Mangifera indica

Application

Application Comment Organism
food industry inhibition of catechol oxidase diminishes browning in Ataulfo mango and elevates food quality Mangifera indica

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol complete inhibition at 0.02 mM Mangifera indica
ascorbate complete inhibition at 2 mM, 94% inhibition at 0.2 mM Mangifera indica
benzoic acid 56% inhibition at 20 mM, no inhibition at 0.02-2.0 mM Mangifera indica
Citric acid complete inhibition at 20 mM, 47% inhibition at 2 mM Mangifera indica
EDTA 73% inhibition at 2 mM, 31% inhibition at 0.2 mM Mangifera indica
glutathione complete inhibition at 2 mM, 94% inhibition at 0.2 mM Mangifera indica
kojic acid 96% inhibition at 2 mM, 60% inhibition at 0.2 mM Mangifera indica
meta-bisulfite complete inhibition at 0.2 mM, 76% inhibition at 0.02 mM Mangifera indica
NaCl 54% inhibition at 2 mM, 19% inhibition at 0.2 mM Mangifera indica
Sodium azide complete inhibition at 2 mM, 99% inhibition at 0.2 mM Mangifera indica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics at different pH and in different buffer systems, overview Mangifera indica
0.41
-
 catechol pH 6.2, 25°C, sodium phosphate buffer Mangifera indica
0.68
-
 catechol pH 6.0, 25°C, MES buffer Mangifera indica
0.79
-
 pyrogallol pH 6.0, 25°C, MES buffer Mangifera indica
0.83
-
 pyrogallol pH 5.8, 25°C, sodium phosphate buffer Mangifera indica
0.87
-
 catechol pH 5.8, 25°C, sodium phosphate buffer Mangifera indica
1.25
-
 catechol pH 5.8, 25°C, MES buffer Mangifera indica
1.26
-
 pyrogallol pH 5.4, 25°C, MES buffer Mangifera indica
1.31
-
 3-methylcatechol pH 5.6, 25°C, MES buffer Mangifera indica
3.69
-
 3-methylcatechol pH 5.4, 25°C, sodium acetate buffer Mangifera indica
4.6
-
 gallate pH 5.8, 25°C Mangifera indica
7.31
-
 pyrogallol pH 5.6, 25°C, sodium acetate buffer Mangifera indica

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper-containing enzyme Mangifera indica

Organism

Organism UniProt Comment Textmining
Mangifera indica D2XZ13
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit PPO activity is higher in the skin compared to the pulp and increases with ripeness Mangifera indica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 3-methylcatechol + O2
-
 Mangifera indica 2 3-methyl-1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2
-
 Mangifera indica 2 1,2-benzoquinone + 2 H2O
-
 ?
gallate + O2
-
 Mangifera indica ?
-
 ?
additional information substrate specificity, overview Mangifera indica ?
-
 ?
protocatechuate + O2
-
 Mangifera indica ?
-
 ?
pyrogallol + O2
-
 Mangifera indica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 144000 + x * 112000 + x * 72000 + x * 23000, SDS-PAGE, differently distributed in fruit pulp and skin, some might by degeneration products Mangifera indica

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Mangifera indica
PPO
-
 Mangifera indica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Mangifera indica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 30 min, 50% activity remaining Mangifera indica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4 6.4 dependent on the substrate, overview Mangifera indica