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Literature summary for 1.10.3.1 extracted from

  • Pinto, M.S.; Siqueira, F.P.; Oliveira, A.E.; Fernandes, K.V.
    A wounding-induced PPO from cowpea (Vigna unguiculata) seedlings (2008), Phytochemistry, 69, 2297-2302.
    View publication on PubMed
Search for more literature for 1.10.3.1

Activating Compound

Activating Compound Comment Organism Structure
SDS enzyme is optimally activated by 10 mM SDS Vigna unguiculata

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, 15% inhibition Vigna unguiculata
resorcinol 10 mM, 40% inhibition Vigna unguiculata
Sodium azide 10 mM, 100% inhibition Vigna unguiculata
tropolone most powerful specific PPO inhibitor. It reduces the PPO activity by 50%, when used at a low 0.01 mM concentration Vigna unguiculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.44
-
 4-methylcatechol
-
 Vigna unguiculata
9.86
-
 4-methylcatechol
-
 Vigna unguiculata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected Vigna unguiculata
73000
-
 by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected Vigna unguiculata
220000
-
 by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected Vigna unguiculata

Organism

Organism UniProt Comment Textmining
Vigna unguiculata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using (NH4)2SO4 precipitation and ion-exchange chromatography Vigna unguiculata

Source Tissue

Source Tissue Comment Organism Textmining
seedling polyphenol oxidases are induced in cowpea plants by wounding Vigna unguiculata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 polyphenol oxidases are induced in cowpea plants by wounding. The highest activity levels are detected 48 h after this stimulus in both wounded and neighbor-to-wounded unifoliates of cowpea seedlings. The increase of activity is in the order of 13 to 15fold, respectively, in comparison to control unifoliates Vigna unguiculata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-catechin + O2 enzyme activity relative to 4-methylcatechol: 0.77% Vigna unguiculata ?
-
 ?
2 catechol + O2 enzyme activity relative to 4-methylcatechol: 87% Vigna unguiculata 2 1,2-benzoquinone + 2 H2O
-
 ?
3-(3,4-dihydroxyphenyl)-L-alanine + O2 enzyme activity relative to 4-methylcatechol: 1.15% Vigna unguiculata ?
-
 ?
4-methylcatechol + O2 enzyme shows highest activity with 4-methylcatechol Vigna unguiculata ?
-
 ?
chlorogenic acid + O2 enzyme activity relative to 4-methylcatechol: 6.15% Vigna unguiculata ?
-
 ?
pyrogallol + O2 enzyme activity relative to 4-methylcatechol: 9.24% Vigna unguiculata ?
-
 ?

Subunits

Subunits Comment Organism
multimer by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected: 58 kDa, 73 kDa and 220 kDa Vigna unguiculata

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Vigna unguiculata
PPO
-
 Vigna unguiculata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 enzyme is highly stable even 5 min at 80°C Vigna unguiculata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Vigna unguiculata
6
-
 optimal pH using 4-methylcatechol as substrate Vigna unguiculata

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8
-
 Vigna unguiculata

General Information

General Information Comment Organism
physiological function polyphenol oxidases are induced in cowpea plants by wounding Vigna unguiculata