KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | general kinetic mechanism, the diphenolase activity is characterized by a lag period, whose duration depends on the substrate concentration, the pH, and the presence of catalytic amounts of o-diphenol, overview | Cydonia oblonga | |
1.2 | - |
4-methylcatechol | pH 7.0, 25°C | Cydonia oblonga |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cydonia oblonga | - |
var. Gigante de Vranja, quince | - |
Purification (Comment) | Organism |
---|---|
partially purified in a sequential two-phase system based on Triton X-114 and PEG-8000/phosphate, followed by ammonium sulfate fractionation | Cydonia oblonga |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Cydonia oblonga | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay optimization | Cydonia oblonga |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + O2 | - |
Cydonia oblonga | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
catechol + 1/2 O2 | - |
Cydonia oblonga | 1,2-benzoquinone + H2O | - |
? | |
additional information | the enzyme catalyzes the hydroxylation of monophenols to o-diphenols, monophenolase activity EC 1.14.18.1, and the oxidation of the o-diphenols to o-quinones, diphenolase activity EC 1.10.3.1, cross-reaction analysis, overview | Cydonia oblonga | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
catecholase | - |
Cydonia oblonga |
dihydroxy-L-phenylalanine:oxygen oxidoreductase | - |
Cydonia oblonga |
diphenolase | - |
Cydonia oblonga |
More | cf. 1.14.18.1 | Cydonia oblonga |
polyphenol oxidase | - |
Cydonia oblonga |
PPO | - |
Cydonia oblonga |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cydonia oblonga |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Cydonia oblonga |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
catecholase activity pH profile | Cydonia oblonga |