Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Rhaphiolepis bibas | |
ascorbate | - |
Rhaphiolepis bibas | |
DTT | - |
Rhaphiolepis bibas | |
glutathione | - |
Rhaphiolepis bibas | |
L-cysteine | - |
Rhaphiolepis bibas | |
additional information | sensitivity to inhibitors of the soluble and particulate enzyme forms, overview | Rhaphiolepis bibas | |
NaCl | - |
Rhaphiolepis bibas | |
NaF | - |
Rhaphiolepis bibas | |
Sodium diethyl dithiocarbamate | - |
Rhaphiolepis bibas | |
sodium disulfite | - |
Rhaphiolepis bibas | |
Thiourea | - |
Rhaphiolepis bibas | |
tropolone | - |
Rhaphiolepis bibas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | temperature and pH dependencis of the two enzyme forms, in absence or presence of SDS | Rhaphiolepis bibas | |
1 | - |
chlorogenic acid | pH 4.5, 25°C, soluble, active enzyme form | Rhaphiolepis bibas | |
1.2 | - |
tert-butyl-catechol | pH 4.5, 25°C, soluble, active enzyme form | Rhaphiolepis bibas | |
1.23 | - |
tert-butyl-catechol | pH 4.5, 25°C, particulate, latent enzyme form | Rhaphiolepis bibas | |
5.7 | - |
chlorogenic acid | pH 4.5, 25°C, particulate, latent enzyme form | Rhaphiolepis bibas |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
particle-bound | 80% of total activity | Rhaphiolepis bibas | - |
- |
soluble | 20% of total activity | Rhaphiolepis bibas | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | active site binding | Rhaphiolepis bibas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61200 | - |
particulate, latent enzyme form, gel filtration | Rhaphiolepis bibas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhaphiolepis bibas | - |
cv. Algerie | - |
Purification (Comment) | Organism |
---|---|
native soluble, active enzyme form partially 3.3fold by ammonium sulfate fractionation, particulate, latent active enzyme form 40fold to homogeneity by phase partitioning in Triton X-114 followed by anion exchange and hydrophobic interaction chromatography, and gel filtration | Rhaphiolepis bibas |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Rhaphiolepis bibas | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.7 | - |
partially purified soluble, active enzyme form, substrate tert-butyl-catechol | Rhaphiolepis bibas |
91.5 | - |
purified particulate, latent enzyme form, substrate tert-butyl-catechol | Rhaphiolepis bibas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + O2 | - |
Rhaphiolepis bibas | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
caffeic acid + O2 | - |
Rhaphiolepis bibas | ? | - |
? | |
catechol + 1/2 O2 | - |
Rhaphiolepis bibas | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + O2 | preferred substrate, 50fold faster reaction rate with the particulate, latent enzyme form compared to the soluble active enzyme | Rhaphiolepis bibas | ? | - |
? | |
DL-isoproterenol + O2 | the L-isomer is preferred | Rhaphiolepis bibas | ? | - |
? | |
dopamine + O2 | - |
Rhaphiolepis bibas | ? | - |
? | |
epicatechin + O2 | - |
Rhaphiolepis bibas | ? | - |
? | |
L-dopa + 1/2 O2 | i.e. L-3,4-dihydroxyphenylalanine | Rhaphiolepis bibas | L-dopaquinone + H2O | - |
? | |
additional information | substrate specificity of the soluble and particulate enzyme forms, overview | Rhaphiolepis bibas | ? | - |
? | |
protocatechuic acid + O2 | - |
Rhaphiolepis bibas | ? | - |
? | |
pyrocatechol + O2 | - |
Rhaphiolepis bibas | ? | - |
? | |
tert-butyl-catechol + O2 | 2fold faster reaction rate with the particulate, latent enzyme form compared to the soluble active enzyme | Rhaphiolepis bibas | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 x 59200, particulate, latent enzyme form, SDS-PAGE | Rhaphiolepis bibas |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Rhaphiolepis bibas |
PPO | - |
Rhaphiolepis bibas |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | soluble, active enzyme form | Rhaphiolepis bibas |
70 | - |
particulate, latent enzyme form | Rhaphiolepis bibas |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 70% of maximal activity within at 30-60°C, particulate, latent enzyme form | Rhaphiolepis bibas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | dependent on conditions, overview | Rhaphiolepis bibas |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile, overview | Rhaphiolepis bibas |