Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | competitive with pyrolallol or catechol, noncompetitive with 4-methylcatechol as substrate. IC50: 0.357 mM in reaction with 4-methylcatechol, IC50: 0.818 mM in reaction with pyrogallol, IC50: 0.33 mM in reaction with catechol | Cynara cardunculus var. scolymus | |
DL-dithiothreitol | competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.147 mM in reaction with 4-methylcatechol, IC50: 0.0329 mM in reaction with pyrogallol, IC50: 0.135 mM in reaction with catechol | Cynara cardunculus var. scolymus | |
glutathione | mixed type inhibition with 4-methylcatechol as substrate, noncompetitive with pyrogallol or catechol as substrates. IC50: 0.174 mM in reaction with 4-methylcatechol, IC50: 0.335 mM in reaction with pyrogallol, IC50: 0.323 mM in reaction with catechol | Cynara cardunculus var. scolymus | |
L-Cys | competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.125 mM in reaction with 4-methylcatechol, IC50: 0.637 mM in reaction with pyrogallol, IC50: 0.15 mM in reaction with catechol | Cynara cardunculus var. scolymus | |
additional information | no inhibition by EDTA, 4-aminobenzoate, salicylic acid, gallic acid and benzoic acid | Cynara cardunculus var. scolymus | |
Sodium azide | competitive with 4-methylcatechol or pyrogallol, noncompetitive with catechol as substrate. IC50: 1.31 mM in reaction with 4-methylcatechol, IC50: 10.3 mM in reaction with pyrogallol, IC50: 4.32 mM in reaction with catechol | Cynara cardunculus var. scolymus | |
tropolone | competitive with pyrolallol or catechol, noncompetitive with 4-methylcatechol. IC50: 0.0109 mM in reaction with 4-methylcatechol, IC50: 0.0539 mM in reaction with pyrogallol, IC50: 0.0297 mM in reaction with catechol | Cynara cardunculus var. scolymus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
pyrogallol | - |
Cynara cardunculus var. scolymus | |
10.7 | - |
catechol | - |
Cynara cardunculus var. scolymus | |
11.6 | - |
4-methylcatechol | - |
Cynara cardunculus var. scolymus | |
45 | - |
L-Dopa | - |
Cynara cardunculus var. scolymus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Cynara cardunculus var. scolymus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cynara cardunculus var. scolymus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cynara cardunculus var. scolymus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cynara cardunculus var. scolymus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + O2 | - |
Cynara cardunculus var. scolymus | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
catechol + 1/2 O2 | - |
Cynara cardunculus var. scolymus | 1,2-benzoquinone + H2O | - |
? | |
L-dopa + 1/2 O2 | - |
Cynara cardunculus var. scolymus | L-dopaquinone + H2O | - |
? | |
additional information | no activity detected with L-Tyr, resorcinol and p-cresol | Cynara cardunculus var. scolymus | ? | - |
? | |
pyrogallol + O2 | - |
Cynara cardunculus var. scolymus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Cynara cardunculus var. scolymus |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Cynara cardunculus var. scolymus |
PPO | - |
Cynara cardunculus var. scolymus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature optimum for recation with 4-methylcatechol is below 10°C | Cynara cardunculus var. scolymus |
20 | - |
reaction with pyrogallol | Cynara cardunculus var. scolymus |
40 | - |
reaction with catechol | Cynara cardunculus var. scolymus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
60 min, about 5% loss of activity with pyrogallol. 60 min, about 10% loss of activity with 4-methylcatechol. 60 min, activity with catechol is stable | Cynara cardunculus var. scolymus |
55 | - |
60 min, about 30% loss of activity with pyrogallol and 4-methylcatechol. 60 min, activity with catechol is stable | Cynara cardunculus var. scolymus |
75 | - |
40 min, complete loss of activity with pyrogallol and 4-methylcatechol. 50 min, about 60% loss of activity with catechol, complete inactivation after 60 min | Cynara cardunculus var. scolymus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recation with 4-methylcatechol | Cynara cardunculus var. scolymus |
7 | - |
reaction with catechol | Cynara cardunculus var. scolymus |
8 | - |
reaction with pyrogallol | Cynara cardunculus var. scolymus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Cynara cardunculus var. scolymus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0297 | - |
competitive with pyrolallol or catechol, noncompetitive with 4-methylcatechol. IC50: 0.0109 mM in reaction with 4-methylcatechol, IC50: 0.0539 mM in reaction with pyrogallol, IC50: 0.0297 mM in reaction with catechol | Cynara cardunculus var. scolymus | tropolone | |
0.135 | - |
competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.147 mM in reaction with 4-methylcatechol, IC50: 0.0329 mM in reaction with pyrogallol, IC50: 0.135 mM in reaction with catechol | Cynara cardunculus var. scolymus | DL-dithiothreitol | |
0.15 | - |
competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.125 mM in reaction with 4-methylcatechol, IC50: 0.637 mM in reaction with pyrogallol, IC50: 0.15 mM in reaction with catechol | Cynara cardunculus var. scolymus | L-Cys | |
0.323 | - |
mixed type inhibition with 4-methylcatechol as substrate, noncompetitive with pyrogallol or catechol as substrates. IC50: 0.174 mM in reaction with 4-methylcatechol, IC50: 0.335 mM in reaction with pyrogallol, IC50: 0.323 mM in reaction with catechol | Cynara cardunculus var. scolymus | glutathione | |
0.33 | - |
competitive with pyrolallol or catechol, noncompetitive with 4-methylcatechol as substrate. IC50: 0.357 mM in reaction with 4-methylcatechol, IC50: 0.818 mM in reaction with pyrogallol, IC50: 0.33 mM in reaction with catechol | Cynara cardunculus var. scolymus | ascorbic acid | |
4.32 | - |
competitive with 4-methylcatechol or pyrogallol, noncompetitive with catechol as substrate. IC50: 1.31 mM in reaction with 4-methylcatechol, IC50: 10.3 mM in reaction with pyrogallol, IC50: 4.32 mM in reaction with catechol | Cynara cardunculus var. scolymus | Sodium azide |