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Literature summary for 1.10.3.1 extracted from

  • Dogan, S.; Turan, Y.; Erturk, H.; Arslan, O.
    Characterization and purification of polyphenol oxidase from artichoke (Cynara scolymus L.) (2005), J. Agric. Food Chem., 53, 776-785.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid competitive with pyrolallol or catechol, noncompetitive with 4-methylcatechol as substrate. IC50: 0.357 mM in reaction with 4-methylcatechol, IC50: 0.818 mM in reaction with pyrogallol, IC50: 0.33 mM in reaction with catechol Cynara cardunculus var. scolymus
DL-dithiothreitol competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.147 mM in reaction with 4-methylcatechol, IC50: 0.0329 mM in reaction with pyrogallol, IC50: 0.135 mM in reaction with catechol Cynara cardunculus var. scolymus
glutathione mixed type inhibition with 4-methylcatechol as substrate, noncompetitive with pyrogallol or catechol as substrates. IC50: 0.174 mM in reaction with 4-methylcatechol, IC50: 0.335 mM in reaction with pyrogallol, IC50: 0.323 mM in reaction with catechol Cynara cardunculus var. scolymus
L-Cys competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.125 mM in reaction with 4-methylcatechol, IC50: 0.637 mM in reaction with pyrogallol, IC50: 0.15 mM in reaction with catechol Cynara cardunculus var. scolymus
additional information no inhibition by EDTA, 4-aminobenzoate, salicylic acid, gallic acid and benzoic acid Cynara cardunculus var. scolymus
Sodium azide competitive with 4-methylcatechol or pyrogallol, noncompetitive with catechol as substrate. IC50: 1.31 mM in reaction with 4-methylcatechol, IC50: 10.3 mM in reaction with pyrogallol, IC50: 4.32 mM in reaction with catechol Cynara cardunculus var. scolymus
tropolone competitive with pyrolallol or catechol, noncompetitive with 4-methylcatechol. IC50: 0.0109 mM in reaction with 4-methylcatechol, IC50: 0.0539 mM in reaction with pyrogallol, IC50: 0.0297 mM in reaction with catechol Cynara cardunculus var. scolymus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2
-
pyrogallol
-
Cynara cardunculus var. scolymus
10.7
-
catechol
-
Cynara cardunculus var. scolymus
11.6
-
4-methylcatechol
-
Cynara cardunculus var. scolymus
45
-
L-Dopa
-
Cynara cardunculus var. scolymus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000, SDS-PAGE Cynara cardunculus var. scolymus

Organism

Organism UniProt Comment Textmining
Cynara cardunculus var. scolymus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cynara cardunculus var. scolymus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Cynara cardunculus var. scolymus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
Cynara cardunculus var. scolymus 4-methyl-1,2-benzoquinone + H2O
-
?
catechol + 1/2 O2
-
Cynara cardunculus var. scolymus 1,2-benzoquinone + H2O
-
?
L-dopa + 1/2 O2
-
Cynara cardunculus var. scolymus L-dopaquinone + H2O
-
?
additional information no activity detected with L-Tyr, resorcinol and p-cresol Cynara cardunculus var. scolymus ?
-
?
pyrogallol + O2
-
Cynara cardunculus var. scolymus ?
-
?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Cynara cardunculus var. scolymus

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
Cynara cardunculus var. scolymus
PPO
-
Cynara cardunculus var. scolymus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
temperature optimum for recation with 4-methylcatechol is below 10°C Cynara cardunculus var. scolymus
20
-
reaction with pyrogallol Cynara cardunculus var. scolymus
40
-
reaction with catechol Cynara cardunculus var. scolymus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
60 min, about 5% loss of activity with pyrogallol. 60 min, about 10% loss of activity with 4-methylcatechol. 60 min, activity with catechol is stable Cynara cardunculus var. scolymus
55
-
60 min, about 30% loss of activity with pyrogallol and 4-methylcatechol. 60 min, activity with catechol is stable Cynara cardunculus var. scolymus
75
-
40 min, complete loss of activity with pyrogallol and 4-methylcatechol. 50 min, about 60% loss of activity with catechol, complete inactivation after 60 min Cynara cardunculus var. scolymus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
recation with 4-methylcatechol Cynara cardunculus var. scolymus
7
-
reaction with catechol Cynara cardunculus var. scolymus
8
-
reaction with pyrogallol Cynara cardunculus var. scolymus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Cynara cardunculus var. scolymus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0297
-
competitive with pyrolallol or catechol, noncompetitive with 4-methylcatechol. IC50: 0.0109 mM in reaction with 4-methylcatechol, IC50: 0.0539 mM in reaction with pyrogallol, IC50: 0.0297 mM in reaction with catechol Cynara cardunculus var. scolymus tropolone
0.135
-
competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.147 mM in reaction with 4-methylcatechol, IC50: 0.0329 mM in reaction with pyrogallol, IC50: 0.135 mM in reaction with catechol Cynara cardunculus var. scolymus DL-dithiothreitol
0.15
-
competitive with 4-methylcatechol, catechol or pyrogallol. IC50: 0.125 mM in reaction with 4-methylcatechol, IC50: 0.637 mM in reaction with pyrogallol, IC50: 0.15 mM in reaction with catechol Cynara cardunculus var. scolymus L-Cys
0.323
-
mixed type inhibition with 4-methylcatechol as substrate, noncompetitive with pyrogallol or catechol as substrates. IC50: 0.174 mM in reaction with 4-methylcatechol, IC50: 0.335 mM in reaction with pyrogallol, IC50: 0.323 mM in reaction with catechol Cynara cardunculus var. scolymus glutathione
0.33
-
competitive with pyrolallol or catechol, noncompetitive with 4-methylcatechol as substrate. IC50: 0.357 mM in reaction with 4-methylcatechol, IC50: 0.818 mM in reaction with pyrogallol, IC50: 0.33 mM in reaction with catechol Cynara cardunculus var. scolymus ascorbic acid
4.32
-
competitive with 4-methylcatechol or pyrogallol, noncompetitive with catechol as substrate. IC50: 1.31 mM in reaction with 4-methylcatechol, IC50: 10.3 mM in reaction with pyrogallol, IC50: 4.32 mM in reaction with catechol Cynara cardunculus var. scolymus Sodium azide