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Literature summary for 1.1.99.B6 extracted from

  • Shao, Z.; Blodgett, J.A.V.; Circello, B.T.; Eliot, A.C.; Woodyer, R.; Li, G.; van der Donk, W.A.; Metcalf, W.W.; Zhao, H.
    Biosynthesis of 2-hydroxyethylphosphonate, an unexpected intermediate common to multiple phosphonate biosynthetic pathways (2008), J. Biol. Chem., 283, 23161-23168.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.B6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells as His6-tagged enzyme Streptomyces wedmorensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0098
-
 NADPH in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces wedmorensis
0.031
-
 phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces wedmorensis

Organism

Organism UniProt Comment Textmining
Streptomyces wedmorensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon immobilized metal affinity chromatography and Superdex 200 gel filtration Streptomyces wedmorensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyethylphosphonate + NADP+
-
 Streptomyces wedmorensis phosphonoacetaldehyde + NADPH + H+
-
 r
additional information no reaction is observed with hydroxymethylphosphonate and 3-hydroxypropylphosphonate Streptomyces wedmorensis ?
-
 ?

Synonyms

Synonyms Comment Organism
FomC
-
 Streptomyces wedmorensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces wedmorensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 phosphonoacetaldehyde reduction is more favorable under neutral conditions (optimal pH at 7.0) Streptomyces wedmorensis
9
-
 2-hydroxyethylphosphonate oxidation is favored under basic conditions (optimal pH at 9.0) Streptomyces wedmorensis

Cofactor

Cofactor Comment Organism Structure
NADPH FomC is an NADPH-dependent enzyme Streptomyces wedmorensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
41
-
 phosphonoacetaldehyde in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication Streptomyces wedmorensis