A38V

the mutation significantly increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

A38V/S388N

the mutation increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

E247A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Q353A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Q353N

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Q384A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Q384N

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

W351A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

W351A

the mutation increases Km values by up to 2 orders of magnitude while also increasing kcat up to 3fold on cello- and xylo-oligosaccharides and showing no substrate inhibition compared to the wild type enzyme

Sarocladium strictum

Y72A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Y72F

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

0.005

xylohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.039

cellobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.04

cellobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.05

cellotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.05

xylopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.05

cellotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.05

xylobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.05

xylopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.05

cellobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.052

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.057

xylobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.057

cellopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.059

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.06

cellopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.06

xylohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.06

cellotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.067

xylotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.07

cellotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.07

xylotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.078

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.08

xylotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.08

cellohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.08

xylotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.08

cellohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.08

cellotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.09

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.09

cellotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.09

cellopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.099

xylobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.1

xylotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.1

cellohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.1

xylohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.104

xylopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.11

xylotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.3

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

12

D-glucose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

15

D-glucose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

15

D-glucose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

118

D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

118

D-xylose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

129

D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

Sarocladium strictum

D7PF15

Sarocladium strictum CBS 346.70

D7PF15

glycoprotein

Sarocladium strictum

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum CBS 346.70

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum CBS 346.70

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum CBS 346.70

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

cellobiose + O2

740204

Sarocladium strictum

cellobiono-1,5-lactone + H2O2

?

cellobiose + O2

740204

Sarocladium strictum CBS 346.70

cellobiono-1,5-lactone + H2O2

?

cellohexaose + O2

740204

Sarocladium strictum

cellohexaono-1,5-lactone + H2O2

?

cellopentaose + O2

740204

Sarocladium strictum

cellopentaono-1,5-lactone + H2O2

?

cellotetraose + O2

740204

Sarocladium strictum

cellotetraono-1,5-lactone + H2O2

?

cellotriose + O2

740204

Sarocladium strictum

cellotriono-1,5-lactone + H2O2

?

D-glucose + O2

740204

Sarocladium strictum

D-glucono-1,5-lactone + H2O2

?

D-glucose + O2

740204

Sarocladium strictum CBS 346.70

D-glucono-1,5-lactone + H2O2

?

D-xylose + O2

740204

Sarocladium strictum

D-xylono-1,5-lactone + H2O2

?

xylobiose + O2

highest activity

740204

Sarocladium strictum

xylobiono-1,5-lactone + H2O2

?

xylohexaose + O2

740204

Sarocladium strictum

xylohexaono-1,5-lactone + H2O2

?

xylopentaose + O2

740204

Sarocladium strictum

xylopentaono-1,5-lactone + H2O2

?

xylotetraose + O2

740204

Sarocladium strictum

xylotetraono-1,5-lactone + H2O2

?

xylotriose + O2

740204

Sarocladium strictum

xylotriono-1,5-lactone + H2O2

?

gluco-oligosaccharide oxidase

Sarocladium strictum

GOOX

Sarocladium strictum

5.5

D-xylose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

6

D-glucose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

6.2

cellobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

6.3

D-glucose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

6.6

cellohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

6.9

cellotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

7

cellobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

7.2

cellohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

7.3

cellotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

7.4

D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

7.5

xylobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

7.6

cellopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

7.8

cellotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

7.8

xylohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

8.1

xylotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

8.3

cellopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

8.6

xylohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

8.7

xylobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

8.7

xylotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

8.8

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

8.8

xylopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

9

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

9.7

xylotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

9.9

cellotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

10.3

xylotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

10.4

xylopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

10.4

D-glucose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

11

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

12

cellobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

12.3

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

12.4

D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

12.7

cellopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

12.8

cellotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

13.2

cellohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

13.5

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

13.7

cellotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

14.5

xylotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

14.8

xylobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

15.2

xylopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

15.2

xylohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

16

xylotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

FAD

Sarocladium strictum

FAD

Sarocladium strictum

A38V

the mutation significantly increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

A38V/S388N

the mutation increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

E247A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Q353A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Q353N

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Q384A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Q384N

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

W351A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

W351A

the mutation increases Km values by up to 2 orders of magnitude while also increasing kcat up to 3fold on cello- and xylo-oligosaccharides and showing no substrate inhibition compared to the wild type enzyme

Sarocladium strictum

Y72A

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

Y72F

the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme

Sarocladium strictum

0.005

xylohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.039

cellobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.04

cellobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.05

cellotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.05

xylopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.05

cellotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.05

xylobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.05

xylopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.05

cellobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.052

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.057

xylobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.057

cellopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.059

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.06

cellopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.06

xylohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.06

cellotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.067

xylotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.07

cellotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.07

xylotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.078

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.08

xylotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.08

cellohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.08

xylotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.08

cellohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.08

cellotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.09

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.09

cellotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.09

cellopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.099

xylobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.1

xylotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.1

cellohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.1

xylohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.104

xylopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.11

xylotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.3

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

12

D-glucose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

15

D-glucose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

15

D-glucose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

118

D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

118

D-xylose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

129

D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

glycoprotein

Sarocladium strictum

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum CBS 346.70

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum CBS 346.70

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2

740204

Sarocladium strictum CBS 346.70

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2

?

cellobiose + O2

740204

Sarocladium strictum

cellobiono-1,5-lactone + H2O2

?

cellobiose + O2

740204

Sarocladium strictum CBS 346.70

cellobiono-1,5-lactone + H2O2

?

cellohexaose + O2

740204

Sarocladium strictum

cellohexaono-1,5-lactone + H2O2

?

cellopentaose + O2

740204

Sarocladium strictum

cellopentaono-1,5-lactone + H2O2

?

cellotetraose + O2

740204

Sarocladium strictum

cellotetraono-1,5-lactone + H2O2

?

cellotriose + O2

740204

Sarocladium strictum

cellotriono-1,5-lactone + H2O2

?

D-glucose + O2

740204

Sarocladium strictum

D-glucono-1,5-lactone + H2O2

?

D-glucose + O2

740204

Sarocladium strictum CBS 346.70

D-glucono-1,5-lactone + H2O2

?

D-xylose + O2

740204

Sarocladium strictum

D-xylono-1,5-lactone + H2O2

?

xylobiose + O2

highest activity

740204

Sarocladium strictum

xylobiono-1,5-lactone + H2O2

?

xylohexaose + O2

740204

Sarocladium strictum

xylohexaono-1,5-lactone + H2O2

?

xylopentaose + O2

740204

Sarocladium strictum

xylopentaono-1,5-lactone + H2O2

?

xylotetraose + O2

740204

Sarocladium strictum

xylotetraono-1,5-lactone + H2O2

?

xylotriose + O2

740204

Sarocladium strictum

xylotriono-1,5-lactone + H2O2

?

5.5

D-xylose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

6

D-glucose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

6.2

cellobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

6.3

D-glucose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

6.6

cellohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

6.9

cellotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

7

cellobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

7.2

cellohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

7.3

cellotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

7.4

D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

7.5

xylobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

7.6

cellopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

7.8

cellotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

7.8

xylohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

8.1

xylotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

8.3

cellopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

8.6

xylohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

8.7

xylobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

8.7

xylotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

8.8

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

8.8

xylopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

9

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

9.7

xylotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

9.9

cellotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

10.3

xylotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

10.4

xylopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

10.4

D-glucose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

11

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

12

cellobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

12.3

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

12.4

D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

12.7

cellopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

12.8

cellotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

13.2

cellohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

13.5

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

13.7

cellotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

14.5

xylotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

14.8

xylobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

15.2

xylopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

15.2

xylohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

16

xylotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.05

D-xylose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.06

D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.11

D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.4

D-glucose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.42

D-glucose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.83

D-glucose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

36.5

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

66.7

cellohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

73.3

xylotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

73.3

xylohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

75

xylobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

78.3

cellopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

85

xylotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

85

xylopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

86.7

cellotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

91.7

cellohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

93.3

cellotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

115

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

116.7

cellobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

135

xylohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

136.7

xylotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

136.7

xylotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

143.3

cellotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

145

cellopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

148.3

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

150

cellotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

150

cellohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

150

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

151.7

xylobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

156.7

cellobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

183.3

xylotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

196.7

xylopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

216.7

cellopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

233.3

cellotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

233.3

xylotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

233.3

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

250

cellotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

283.3

xylopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

300

xylobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

300

xylohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

306.7

cellobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.05

D-xylose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.06

D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.11

D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

0.4

D-glucose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

0.42

D-glucose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

0.83

D-glucose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

36.5

4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

66.7

cellohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

73.3

xylotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

73.3

xylohexaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

75

xylobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

78.3

cellopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

85

xylotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

85

xylopentaose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

86.7

cellotetraose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

91.7

cellohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

93.3

cellotriose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

115

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

116.7

cellobiose

mutant enzyme A38V/S388N, at pH 8.0 and 37°C

Sarocladium strictum

135

xylohexaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

136.7

xylotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

136.7

xylotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

143.3

cellotriose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

145

cellopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

148.3

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

150

cellotetraose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

150

cellohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

150

alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

151.7

xylobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

156.7

cellobiose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

183.3

xylotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

196.7

xylopentaose

wild type enzyme, at pH 8.0 and 37°C

Sarocladium strictum

216.7

cellopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

233.3

cellotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

233.3

xylotetraose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

233.3

alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

250

cellotriose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

283.3

xylopentaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

300

xylobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

300

xylohexaose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

306.7

cellobiose

mutant enzyme A38V, at pH 8.0 and 37°C

Sarocladium strictum

741429

Vuong

Direct comparison of gluco-oli ...

Sci. Rep.

743824

Vuong

Direct comparison of gluco-ol ...

Sci. Rep.

741237

Foumani

Enhanced polysaccharide bindin ...

PLoS ONE

741305

Vuong

Fusion of a xylan-binding modu ...

PLoS ONE

724616

Foumani

Altered substrate specificity ...

Biotechnol. Bioeng.

685689

Kiryu

Purification and characterizat ...

Biosci. Biotechnol. Biochem.

698829

Huang

Functional roles of the 6-S-cy ...

J. Biol. Chem.