Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Sarocladium strictum |
Protein Variants | Comment | Organism |
---|---|---|
W351F | the mutant shows reduced kcat values for monosaccharide and oligosaccharide substrates | Sarocladium strictum |
Y300A | the mutation doubles kcat values for monosaccharide and oligosaccharide substrates | Sarocladium strictum |
Y300N | the mutation doubles kcat values for monosaccharide and oligosaccharide substrates | Sarocladium strictum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
cellobiose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
0.083 | - |
cellobiose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
0.085 | - |
cellotriose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
0.098 | - |
xylobiose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
0.1 | - |
xylotriose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
0.11 | - |
cellotriose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
0.25 | - |
cellobiose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
0.25 | - |
cellotriose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
0.31 | - |
xylotriose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
0.35 | - |
xylobiose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
0.38 | - |
cellobiose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
0.44 | - |
cellotriose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
2.81 | - |
maltose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
3.11 | - |
D-glucose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
3.15 | - |
xylotriose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
4.3 | - |
xylotriose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
4.83 | - |
xylobiose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
5 | - |
maltose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
5.11 | - |
xylobiose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
8.1 | - |
D-glucose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
11 | - |
maltose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
17.4 | - |
D-glucose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
19.6 | - |
maltose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
31 | - |
D-glucose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
32 | - |
D-xylose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
36.1 | - |
D-galactose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
51.8 | - |
D-xylose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
56 | - |
N-acetyl-D-glucosamine | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
92 | - |
N-acetyl-D-glucosamine | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
96 | - |
D-galactose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
105 | - |
D-xylose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
110 | - |
D-galactose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
132 | - |
D-galactose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
288 | - |
D-xylose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
340 | - |
N-acetyl-D-glucosamine | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
950 | - |
N-acetyl-D-glucosamine | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + O2 | Sarocladium strictum | - |
? + H2O2 | - |
? | |
cellobiose + O2 | Sarocladium strictum CBS 346.70 | - |
? + H2O2 | - |
? | |
cellotriose + O2 | Sarocladium strictum | - |
? + H2O2 | - |
? | |
cellotriose + O2 | Sarocladium strictum CBS 346.70 | - |
? + H2O2 | - |
? | |
D-galactose + O2 | Sarocladium strictum | - |
? + H2O2 | - |
? | |
D-glucose + O2 | Sarocladium strictum | highest catalytic efficiency with D-glucose | ? + H2O2 | - |
? | |
D-xylose + O2 | Sarocladium strictum | - |
? + H2O2 | - |
? | |
D-xylose + O2 | Sarocladium strictum CBS 346.70 | - |
? + H2O2 | - |
? | |
maltose + O2 | Sarocladium strictum | - |
? + H2O2 | - |
? | |
maltose + O2 | Sarocladium strictum CBS 346.70 | - |
? + H2O2 | - |
? | |
additional information | Sarocladium strictum | no activity with mannose | ? | - |
? | |
additional information | Sarocladium strictum CBS 346.70 | no activity with mannose | ? | - |
? | |
N-acetyl-D-glucosamine + O2 | Sarocladium strictum | - |
? + H2O2 | - |
? | |
xylobiose + O2 | Sarocladium strictum | - |
? + H2O2 | - |
? | |
xylotriose + O2 | Sarocladium strictum | - |
? + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sarocladium strictum | Q6PW77 | - |
- |
Sarocladium strictum CBS 346.70 | Q6PW77 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Sarocladium strictum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:wheat bran-grown cell | - |
Sarocladium strictum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + O2 | - |
Sarocladium strictum | ? + H2O2 | - |
? | |
cellobiose + O2 | - |
Sarocladium strictum CBS 346.70 | ? + H2O2 | - |
? | |
cellotriose + O2 | - |
Sarocladium strictum | ? + H2O2 | - |
? | |
cellotriose + O2 | - |
Sarocladium strictum CBS 346.70 | ? + H2O2 | - |
? | |
D-galactose + O2 | - |
Sarocladium strictum | ? + H2O2 | - |
? | |
D-glucose + O2 | highest catalytic efficiency with D-glucose | Sarocladium strictum | ? + H2O2 | - |
? | |
D-xylose + O2 | - |
Sarocladium strictum | ? + H2O2 | - |
? | |
D-xylose + O2 | - |
Sarocladium strictum CBS 346.70 | ? + H2O2 | - |
? | |
maltose + O2 | - |
Sarocladium strictum | ? + H2O2 | - |
? | |
maltose + O2 | - |
Sarocladium strictum CBS 346.70 | ? + H2O2 | - |
? | |
additional information | no activity with mannose | Sarocladium strictum | ? | - |
? | |
additional information | no activity with mannose | Sarocladium strictum CBS 346.70 | ? | - |
? | |
N-acetyl-D-glucosamine + O2 | - |
Sarocladium strictum | ? + H2O2 | - |
? | |
xylobiose + O2 | - |
Sarocladium strictum | ? + H2O2 | - |
? | |
xylotriose + O2 | - |
Sarocladium strictum | ? + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gluco-oligosaccharide oxidase | - |
Sarocladium strictum |
GOOX | - |
Sarocladium strictum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
D-xylose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
5.3 | - |
cellotriose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
5.3 | - |
D-xylose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
5.4 | - |
maltose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
5.6 | - |
D-glucose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
5.7 | - |
cellobiose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
6 | - |
maltose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
6 | - |
cellotriose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
6.3 | - |
cellobiose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
6.6 | - |
D-galactose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
7.2 | - |
D-galactose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
7.5 | - |
D-glucose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
7.8 | - |
N-acetyl-D-glucosamine | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
7.9 | - |
xylotriose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
8 | - |
xylobiose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
8.1 | - |
N-acetyl-D-glucosamine | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
8.3 | - |
xylotriose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
8.8 | - |
xylobiose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
10 | - |
cellotriose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
10.4 | - |
maltose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
10.4 | - |
maltose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
10.8 | - |
D-glucose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
11.2 | - |
cellotriose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
11.3 | - |
D-xylose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
11.3 | - |
N-acetyl-D-glucosamine | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
11.4 | - |
cellobiose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
11.7 | - |
D-xylose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
11.8 | - |
D-galactose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
12 | - |
xylobiose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
12 | - |
xylotriose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
12.8 | - |
N-acetyl-D-glucosamine | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
13.2 | - |
D-glucose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
13.3 | - |
D-galactose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
13.3 | - |
xylobiose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
13.7 | - |
cellobiose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
13.9 | - |
xylotriose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
N-acetyl-D-glucosamine | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
0.02 | - |
D-xylose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
0.02 | - |
N-acetyl-D-glucosamine | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
0.05 | - |
D-xylose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
0.06 | - |
D-galactose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
0.11 | - |
D-galactose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
0.14 | - |
D-galactose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
0.14 | - |
N-acetyl-D-glucosamine | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
0.18 | - |
D-glucose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
0.18 | - |
D-galactose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
0.2 | - |
N-acetyl-D-glucosamine | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
0.22 | - |
D-xylose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
0.36 | - |
D-xylose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
0.43 | - |
D-glucose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
0.53 | - |
maltose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
0.95 | - |
maltose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
1.1 | - |
maltose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
1.63 | - |
D-glucose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
2.13 | - |
maltose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
2.48 | - |
xylobiose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
2.6 | - |
xylobiose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
2.83 | - |
xylotriose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
3.6 | - |
D-glucose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
4.33 | - |
xylotriose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
23.3 | - |
xylobiose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
23.3 | - |
cellotriose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
25 | - |
xylotriose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
30 | - |
cellobiose | mutant enzyme Y300N, at 37°C and pH 8.0 | Sarocladium strictum | |
45 | - |
cellotriose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
48.3 | - |
cellotriose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
56.7 | - |
cellobiose | mutant enzyme Y300A, at 37°C and pH 8.0 | Sarocladium strictum | |
68.3 | - |
cellobiose | mutant enzyme W351F, at 37°C and pH 8.0 | Sarocladium strictum | |
70 | - |
cellotriose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
85 | - |
xylotriose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
90 | - |
xylobiose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum | |
100 | - |
cellobiose | wild type enzyme, at 37°C and pH 8.0 | Sarocladium strictum |