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Literature summary for 1.1.99.9 extracted from

  • Sundaram, T.K.; Snell, E.E.
    The bacterial oxidation of vitamin B6. V. The enzymatic formation of pyridoxal and isopyridoxal from pyridoxine (1969), J. Biol. Chem., 244, 2577-2584.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by alpha,alpha'-dipyridyl, o-, m- or p-phenanthroline Pseudomonas sp.
p-chloromercuribenzoate
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 pyridoxine
-
 Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 IA
-
Pseudomonas sp. IA
-
 IA
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine + acceptor low activity Pseudomonas sp. 4-aminomethyl-5-formyl-2-methylpyridin-3-ol + reduced acceptor
-
 ?
pyridoxamine + acceptor low activity Pseudomonas sp. IA 4-aminomethyl-5-formyl-2-methylpyridin-3-ol + reduced acceptor
-
 ?
pyridoxine + acceptor highly specific for pyridoxine Pseudomonas sp. isopyridoxal + reduced acceptor
-
 ?
pyridoxine + acceptor not: O2 Pseudomonas sp. isopyridoxal + reduced acceptor
-
 ?
pyridoxine + acceptor acceptor: 2,6-dichlorophenolindophenol Pseudomonas sp. isopyridoxal + reduced acceptor
-
 ?
pyridoxine + acceptor highly specific for pyridoxine Pseudomonas sp. IA isopyridoxal + reduced acceptor
-
 ?
pyridoxine + acceptor not: O2 Pseudomonas sp. IA isopyridoxal + reduced acceptor
-
 ?
pyridoxine + acceptor acceptor: 2,6-dichlorophenolindophenol Pseudomonas sp. IA isopyridoxal + reduced acceptor
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 30% of activity maximum, pH 7.0: 17% of activity maximum Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein, FAD-dependent Pseudomonas sp.