BRENDA - Enzyme Database show
show all sequences of 1.1.99.7

Malate-lactate transhydrogenase from Micrococcus lactilyticus

Allen, S.H.G.; Methods Enzymol. 13, 262-269 (1969)
No PubMed abstract available

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion required
Veillonella parvula
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
99000
100000
sucrose density gradient studies
Veillonella parvula
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-lactate + oxaloacetate
Veillonella parvula
first step in fermentation of lactate
malate + pyruvate
-
Veillonella parvula
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Veillonella parvula
-
formerly named as Micrococcus lactilyticus
-
Purification (Commentary)
Commentary
Organism
DEAE-cellulose, DEAE-Sephadex
Veillonella parvula
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
117
-
-
Veillonella parvula
Storage Stability
Storage Stability
Organism
-15C, as ammonium sulfate suspension, about 10-20 mg of protein per ml, 40% loss of activity after 1 year
Veillonella parvula
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-lactate + oxaloacetate
50% higher specific activity with malate and pyruvate as substrates
287845
Veillonella parvula
malate + pyruvate
-
287845
Veillonella parvula
-
(S)-lactate + oxaloacetate
first step in fermentation of lactate
287845
Veillonella parvula
malate + pyruvate
-
287845
Veillonella parvula
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Veillonella parvula
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.3
9.5
about 50% of maximal activity at pH 6.3 and 9.5
Veillonella parvula
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion required
Veillonella parvula
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
99000
100000
sucrose density gradient studies
Veillonella parvula
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-lactate + oxaloacetate
Veillonella parvula
first step in fermentation of lactate
malate + pyruvate
-
Veillonella parvula
-
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-cellulose, DEAE-Sephadex
Veillonella parvula
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
117
-
-
Veillonella parvula
Storage Stability (protein specific)
Storage Stability
Organism
-15C, as ammonium sulfate suspension, about 10-20 mg of protein per ml, 40% loss of activity after 1 year
Veillonella parvula
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-lactate + oxaloacetate
50% higher specific activity with malate and pyruvate as substrates
287845
Veillonella parvula
malate + pyruvate
-
287845
Veillonella parvula
-
(S)-lactate + oxaloacetate
first step in fermentation of lactate
287845
Veillonella parvula
malate + pyruvate
-
287845
Veillonella parvula
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Veillonella parvula
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.3
9.5
about 50% of maximal activity at pH 6.3 and 9.5
Veillonella parvula
Other publictions for EC 1.1.99.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287848
Allen
Lactate-oxaloacetate transhydr ...
Veillonella parvula
Methods Enzymol.
89
367-376
1982
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-
-
-
-
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-
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-
1
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-
1
-
-
1
1
-
-
1
1
1
1
1
-
-
-
-
-
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2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
1
1
1
1
-
-
-
-
-
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-
-
-
-
-
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-
287847
Allen
Molecular weight and subunit s ...
Veillonella parvula
Eur. J. Biochem.
35
338-345
1973
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-
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-
-
-
-
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1
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-
1
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
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-
-
-
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-
287846
Allen
Studies on the structure and m ...
Veillonella parvula
J. Biol. Chem.
247
909-916
1972
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-
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2
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1
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1
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1
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1
1
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2
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2
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-
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-
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2
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287845
Allen
-
Malate-lactate transhydrogenas ...
Veillonella parvula
Methods Enzymol.
13
262-269
1969
-
-
-
-
-
-
-
-
-
1
1
1
-
1
-
-
1
-
-
-
1
1
2
-
-
-
-
-
1
1
-
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-
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-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
1
1
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287849
Dolin
Kinetics of malic-lactic trans ...
Veillonella parvula
J. Biol. Chem.
244
5273-5285
1969
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-
-
-
-
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7
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-
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-
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1
-
-
-
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1
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-
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7
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-
-
-
-
-
-
-
-
-
-
-
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1
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-
-
-
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-
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-
287850
Dolin
Kinetics of malic-lactic trans ...
Veillonella parvula
J. Biol. Chem.
243
3916-3923
1968
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-
-
-
-
-
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-
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1
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1
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2
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1
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2
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287844
Allen
The isolation and characteriza ...
no activity in Escherichia coli, no activity in Propionibacterium shermanii, Veillonella parvula
J. Biol. Chem.
241
5266-5275
1966
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-
-
-
-
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1
4
-
-
1
1
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4
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-
1
-
-
-
1
-
2
-
1
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
4
-
-
1
1
-
-
-
1
-
-
1
-
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-