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Literature summary for 1.1.99.7 extracted from
- The isolation and characterization of malate-lactate transhydrogenase from Micrococcus lactilyticus (1966), J. Biol. Chem., 241, 5266-5275.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-hydroxymercuribenzoate | - |
Veillonella parvula |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
oxaloacetate | - |
Veillonella parvula | |
| 1.4 | - |
L-malate | - |
Veillonella parvula | |
| 1.9 | - |
L-lactate | - |
Veillonella parvula | |
| 2.4 | - |
pyruvate | - |
Veillonella parvula | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 99000 | 100000 | sucrose density gradient studies | Veillonella parvula |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-lactate + oxaloacetate | Veillonella parvula | - |
malate + pyruvate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Escherichia coli | - |
- |
- |
| no activity in Propionibacterium shermanii | - |
- |
- |
| Veillonella parvula | - |
formerly named as Micrococcus lactilyticus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Veillonella parvula |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 117 | - |
- |
Veillonella parvula |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-lactate + oxaloacetate | - |
Veillonella parvula | malate + pyruvate | - |
r | |
| (S)-lactate + oxaloacetate | catalyzes hydrogen transfer from C3 or C4(S)-2-hydroxy acids to 2-oxo-acids, hydrogen donors: L-malate, DL-lactate and DL-alpha-hydroxybutyrate, low activity with: DL-alpha-hydroxyglutarate, DL-alpha-hydroxyvalerate, DL-beta-hydroxybutyrate and DL-alpha-hydroxycaprylate, hydrogen acceptors: oxalacetate, low activity with: alpha-ketoglutarate, alpha-ketocaprylate | Veillonella parvula | malate + pyruvate | - |
r | |
| pyruvate + L-malate | - |
Veillonella parvula | (S)-lactate + oxaloacetate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Veillonella parvula |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Veillonella parvula |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 9.5 | about 50% of maximal activity at pH 6.3 and 9.5 | Veillonella parvula |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | tightly bound in the active centre | Veillonella parvula | |
| NADH | - |
Veillonella parvula | |
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Release 2023.1 (January 2023)
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