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Literature summary for 1.1.99.6 extracted from
- D-2-hydroxy acid dehydrogenase from animal tissue (1975), Methods Enzymol., 41, 323-329.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ferricyanide | 100 mM, 2.2fold increase in enzyme activity | Oryctolagus cuniculus |  |
| methylene blue | - |
Oryctolagus cuniculus | |
| additional information | NAD+ or NADP+ do not act as acceptors | Oryctolagus cuniculus | |
| O2 | possible physiological acceptor | Oryctolagus cuniculus | |
General Stability
| General Stability | Organism |
|---|---|
| oxalate stabilizes | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | inactivation during preincubation at 0°C; progressive inactivation, substrate decreases rate of inactivation | Oryctolagus cuniculus | |
| EDTA | progressive inactivation, substrate decreases rate of inactivation | Oryctolagus cuniculus | |
| KCN | competitive, reversible | Oryctolagus cuniculus | |
| L-lactate | weak | Oryctolagus cuniculus | |
| oxalate | competitive | Oryctolagus cuniculus | |
| oxaloacetate | competitive to acceptor | Oryctolagus cuniculus | |
| pyruvate | competitive inhibitor to acceptor | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
D-malate | - |
Oryctolagus cuniculus | |
| 2 | - |
D-lactate | - |
Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| particle-bound | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 102000 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + acceptor | Oryctolagus cuniculus | enzyme acts on a variety of (R)-2-hydroxy acids | pyruvate + reduced acceptor | - |
? | |
| (R)-lactate + acceptor | Oryctolagus cuniculus | cytochrome c, ubiquinone and O2 are possible physiological electron acceptors | pyruvate + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, as precipitate in ammonium sulfate stable for months | Oryctolagus cuniculus |
| 4°C, in 25 mM Tris buffer, pH 8.0, stable for weeks | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + acceptor | cytochrome c, ubiquinone and O2 are possible physiological acceptors | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
| (R)-lactate + acceptor | no activity with glycolate, 3-methylpentanoate, phenylglycolate, phenyllactate, 3-phosphoglycerate, 6-phosphoglycerate | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
| (R)-lactate + acceptor | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
| (R)-lactate + acceptor | enzyme acts on a variety of (R)-2-hydroxy acids | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
| (R)-lactate + acceptor | cytochrome c, ubiquinone and O2 are possible physiological electron acceptors | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
| 2(R)-hydroxybutanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxobutanoate + reduced acceptor | - |
? | |
| 2(R)-hydroxyheptanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxoheptanoate + reduced acceptor | - |
? | |
| 2(R)-hydroxyhexanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxohexanoate + reduced acceptor | - |
? | |
| 2(R)-hydroxyoctanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxooctanoate + reduced acceptor | - |
? | |
| 2(R)-hydroxypentanoate + acceptor | - |
Oryctolagus cuniculus | 2-oxopentanoate + reduced acceptor | - |
? | |
| D-glycerate + acceptor | - |
Oryctolagus cuniculus | hydroxypyruvate + reduced acceptor | - |
? | |
| D-malate + acceptor | - |
Oryctolagus cuniculus | oxaloacetate + reduced acceptor | - |
? | |
| D-tartrate + acceptor | poor substrate | Oryctolagus cuniculus | 2-oxo-3-hydroxybutanoate + reduced acceptor | - |
? | |
| meso-tartrate + acceptor | good substrate | Oryctolagus cuniculus | 2-oxo-3-hydroxybutanoate + reduced acceptor | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
and below for malate as substrate | Oryctolagus cuniculus |
| 8.6 | - |
D-lactate as substrate | Oryctolagus cuniculus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
in 25 mM Tris buffer at 4°C stable for weeks | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | - |
Oryctolagus cuniculus | |
| cytochrome c | possible physiological acceptor | Oryctolagus cuniculus | |
| FAD | flavoprotein, 2 molecules per molecule of enzyme, not replaceable by FMN or riboflavin | Oryctolagus cuniculus | |
| phenazine methosulfate | - |
Oryctolagus cuniculus | |
| ubiquinone | possible physiological acceptor | Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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