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Literature summary for 1.1.99.42 extracted from

  • Ge, F.; Yokochi, N.; Yoshikane, Y.; Ohnishi, K.; Yagi, T.
    Gene identification and characterization of the pyridoxine degradative enzyme 4-pyridoxic acid dehydrogenase from the nitrogen-fixing symbiotic bacterium Mesorhizobium loti MAFF303099 (2008), J. Biochem., 143, 603-609 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
4-pyridoxate pH 8.0, temperature not specified in the publication Mesorhizobium loti

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Mesorhizobium loti 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
sucrose density centrifugation Mesorhizobium loti
57000
-
gel filtration Mesorhizobium loti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-pyridoxate + 2,6-dichloroindophenol Mesorhizobium loti
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol
-
?
4-pyridoxate + 2,6-dichloroindophenol Mesorhizobium loti MAFF303099
-
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol
-
?

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti Q988C9
-
-
Mesorhizobium loti MAFF303099 Q988C9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.9
-
pH 8.0, temperature not specified in the publication Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-pyridoxate + 2,6-dichloroindophenol
-
Mesorhizobium loti 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol
-
?
4-pyridoxate + 2,6-dichloroindophenol
-
Mesorhizobium loti MAFF303099 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol
-
?
additional information very poor activity with substrates pyridoxal, pyridoxine and 4-pyridoxic acid lactone, and simple alpha-hydroxy acids, such as lactic acid and glycolic acid Mesorhizobium loti ?
-
?
additional information very poor activity with substrates pyridoxal, pyridoxine and 4-pyridoxic acid lactone, and simple alpha-hydroxy acids, such as lactic acid and glycolic acid Mesorhizobium loti MAFF303099 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 59000, SDS-PAGE, 1 * 58921, calculated Mesorhizobium loti

Synonyms

Synonyms Comment Organism
mlr6792
-
Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Mesorhizobium loti

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55
-
54% of maximum activity Mesorhizobium loti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable for 10 min Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
4-pyridoxate pH 8.0, temperature not specified in the publication Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Mesorhizobium loti

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol of FAD per mol of subunit. Purified protein is yellowish and shows absorption maxima near 380nm and 455 nm Mesorhizobium loti