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Literature summary for 1.1.99.39 extracted from

  • Zhang, W.; Zhang, M.; Gao, C.; Zhang, Y.; Ge, Y.; Guo, S.; Guo, X.; Zhou, Z.; Liu, Q.; Zhang, Y.; Ma, C.; Tao, F.; Xu, P.
    Coupling between D-3-phosphoglycerate dehydrogenase and D-2-hydroxyglutarate dehydrogenase drives bacterial L-serine synthesis (2017), Proc. Natl. Acad. Sci. USA, 114, E7574-E7582 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged protein Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00771
-
 electron transfer flavoprotein pH 7.4, 37°C Pseudomonas stutzeri
0.17
-
 (R)-2-hydroxyglutarate pH 7.4, 37°C Pseudomonas stutzeri
0.21
-
 cytochrome c pH 7.4, 37°C Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-2-hydroxyglutarate + acceptor Pseudomonas stutzeri
-
 2-oxoglutarate + reduced acceptor
-
 ?
(R)-2-hydroxyglutarate + acceptor Pseudomonas stutzeri A1501
-
 2-oxoglutarate + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
-
-
Pseudomonas stutzeri A1501
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-hydroxyglutarate + acceptor
-
 Pseudomonas stutzeri 2-oxoglutarate + reduced acceptor
-
 ?
(R)-2-hydroxyglutarate + acceptor
-
 Pseudomonas stutzeri A1501 2-oxoglutarate + reduced acceptor
-
 ?
(R)-2-hydroxyglutarate + cytochrome c
-
 Pseudomonas stutzeri 2-oxoglutarate + reduced cytochrome c
-
 ?
(R)-2-hydroxyglutarate + cytochrome c
-
 Pseudomonas stutzeri A1501 2-oxoglutarate + reduced cytochrome c
-
 ?
(R)-2-hydroxyglutarate + electron transfer flavoprotein
-
 Pseudomonas stutzeri 2-oxoglutarate + reduced electron transfer flavoprotein
-
 ?
(R)-2-hydroxyglutarate + electron transfer flavoprotein
-
 Pseudomonas stutzeri A1501 2-oxoglutarate + reduced electron transfer flavoprotein
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Pseudomonas stutzeri

Synonyms

Synonyms Comment Organism
D2HGDH
-
 Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.46
-
 cytochrome c pH 7.4, 37°C Pseudomonas stutzeri
7.9
-
 (R)-2-hydroxyglutarate pH 7.4, 37°C Pseudomonas stutzeri
12.98
-
 electron transfer flavoprotein pH 7.4, 37°C Pseudomonas stutzeri

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme binds one flavin adenine dinucleotide Pseudomonas stutzeri

General Information

General Information Comment Organism
malfunction deletion mutant displays decreased growth. The defect is rescued by adding L-serine Pseudomonas stutzeri
physiological function the enzyme is functionally tied to L-serine synthesis. D-2-Hydroxyglutarate-mediated coupling between D-3-phosphoglycerate dehydrogenase (SerA) and D-2-hydroxyglutarate dehydrogenase drives bacterial L-serine synthesis Pseudomonas stutzeri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.9
-
 cytochrome c pH 7.4, 37°C Pseudomonas stutzeri
45.38
-
 (R)-2-hydroxyglutarate pH 7.4, 37°C Pseudomonas stutzeri
1716.7
-
 electron transfer flavoprotein pH 7.4, 37°C Pseudomonas stutzeri