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Literature summary for 1.1.99.39 extracted from

  • Quaye, J.A.; Gadda, G.
    Kinetic and bioinformatic characterization of D-2-hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa PAO1 (2020), Biochemistry, 59, 4833-4844 .
    View publication on PubMed
Search for more literature for 1.1.99.39

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain Rosetta(DE3)pLysS Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 (S)-2-hydroxyglutarate pH 7.4, 25°C Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-2-hydroxyglutarate + acceptor Pseudomonas aeruginosa necessary step in the serine biosynthetic pathway 2-oxoglutarate + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I6H4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-hydroxyglutarate + phenazine methosulfate
-
 Pseudomonas aeruginosa 2-oxoglutarate + reduced phenazine methosulfate
-
 ?
(S)-2-hydroxyglutarate + acceptor necessary step in the serine biosynthetic pathway Pseudomonas aeruginosa 2-oxoglutarate + reduced acceptor
-
 ?
D-malate + phenazine methosulfate
-
 Pseudomonas aeruginosa ? + reduced phenazine methosulfate
-
 ?

Synonyms

Synonyms Comment Organism
D2HGDH
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
 (S)-2-hydroxyglutarate pH 7.4, 25°C Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
drug target the dependence of Pseudomonas aeruginosa on PaD2HGDH makes the enzyme a potential therapeutic target against Pseudomonas aeruginosa Pseudomonas aeruginosa
evolution a phylogenetic tree analysis of D-2-hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa, vanillyl alcohol oxidase, and human D-2-hydroxyglutarate dehydrogenase establishes genetic diversity among these enzymes Pseudomonas aeruginosa
physiological function the enzyme catalyzes a necessary step in the serine biosynthetic pathway Pseudomonas aeruginosa