BRENDA - Enzyme Database
show all sequences of 1.1.99.38

Electrochemical resolution of the [4Fe-4S] centers of the AdoMet radical enzyme BtrN: evidence of proton coupling and an unusual, low-potential auxiliary cluster

Maiocco, S.J.; Grove, T.L.; Booker, S.J.; Elliott, S.J.; J. Am. Chem. Soc. 137, 8664-8667 (2015)

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Bacillus circulans
Q8G907
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-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine
-
740576
Bacillus circulans
3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine
when AdoMet and 2-deoxy-scyllo-inosamine are bound, reduction of the active site cluster in a pH dependent fashion allows for reductive cleavage of AdoMet, generating a 5'-deoxyadenosine radical,which can then abstract a hydrogen atom from the 2-deoxy-scyllo-inosamine substrate forming a 2-deoxy-scyllo-inosamine radical. The 2-deoxy-scyllo-inosamine radical has an estimated potential of -1.6 V, indicating that it could easily reduce either the auxiliary cluster or the AdoMet radical cluster itself
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?
Cofactor
Cofactor
Commentary
Organism
Structure
[4Fe-4S]-center
the potential of both the AdoMet [4Fe-4S] radical and auxiliary clusters can be measured simultaneously. The AdoMet [4Fe-4S] radical cluster exhibits a midpoint potential of -510 mV, while the auxiliary cluster exhibits a midpoint potential of -765 mV
Bacillus circulans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
[4Fe-4S]-center
the potential of both the AdoMet [4Fe-4S] radical and auxiliary clusters can be measured simultaneously. The AdoMet [4Fe-4S] radical cluster exhibits a midpoint potential of -510 mV, while the auxiliary cluster exhibits a midpoint potential of -765 mV
Bacillus circulans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine
-
740576
Bacillus circulans
3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine
when AdoMet and 2-deoxy-scyllo-inosamine are bound, reduction of the active site cluster in a pH dependent fashion allows for reductive cleavage of AdoMet, generating a 5'-deoxyadenosine radical,which can then abstract a hydrogen atom from the 2-deoxy-scyllo-inosamine substrate forming a 2-deoxy-scyllo-inosamine radical. The 2-deoxy-scyllo-inosamine radical has an estimated potential of -1.6 V, indicating that it could easily reduce either the auxiliary cluster or the AdoMet radical cluster itself
-
-
?
Other publictions for EC 1.1.99.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740576
Maiocco
Electrochemical resolution of ...
Bacillus circulans
J. Am. Chem. Soc.
137
8664-8667
2015
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741329
Goldman
X-ray analysis of butirosin bi ...
Bacillus circulans
Proc. Natl. Acad. Sci. USA
110
15949-15954
2013
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718869
Grove
A consensus mechanism for radi ...
Bacillus circulans
Biochemistry
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3783-3785
2010
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685280
Yokoyama
Mechanistic study on the react ...
Bacillus circulans
Biochemistry
47
8950-8960
2008
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687282
Yokoyama
Characterization and mechanist ...
Bacillus circulans
J. Am. Chem. Soc.
129
15147-15155
2007
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