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Literature summary for 1.1.99.38 extracted from

  • Yokoyama, K.; Numakura, M.; Kudo, F.; Ohmori, D.; Eguchi, T.
    Characterization and mechanistic study of a radical SAM dehydrogenase in the biosynthesis of butirosin (2007), J. Am. Chem. Soc., 129, 15147-15155.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Niallia circulans

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-scyllo-inosamine substrate inhibition Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
2-deoxy-scyllo-inosamine pH 8.0, 28┬░C Niallia circulans
0.46
-
S-adenosyl-L-methionine pH 8.0, 28┬░C Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans Q8G907
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine enzyme catalyzes the oxidation of 2-deoxy-scyllo-inosamine under strictly anaerobic conditions along with consumption of an equimolar amount of S-adenosyl-L-methionine to produce 5-deoxyadenosine, methionine, and 3-amino-2,3-dideoxy-scylloinososeReaction follows the ordered Bi Ter mechanism and S-adenosyl-L-methionine is the first substrate and 2-deoxy-scyllo-inosamine is the second. The hydrogen atom at C-3 of 2-deoxy-scyllo-inosamine is directly transferred to 5-deoxyadenosine to give the radical intermediate of 2-deoxy-scyllo-inosamine in a reversible hydrogen abstraction step Niallia circulans 3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine
-
?
2-deoxystreptamine + S-adenosyl-L-methionine 14.4% of the rate with 2-deoxy-scyllo-inosamine Niallia circulans ? + 5'-deoxyadenosine + L-methionine
-
?
additional information no substrates: 1D-chiro-inositol, 1L-chiro-inositol, muco-inositol, allo-inositol, D-glucose, D-glucosamine, D-xylose Niallia circulans ?
-
?
myo-inositol + S-adenosyl-L-methionine 0.9% of the rate with 2-deoxy-scyllo-inosamine Niallia circulans ? + 5'-deoxyadenosine + L-methionine
-
?
scyllo-inositol + S-adenosyl-L-methionine 3.3% of the rate with 2-deoxy-scyllo-inosamine Niallia circulans ? + 5'-deoxyadenosine + L-methionine
-
?

Synonyms

Synonyms Comment Organism
BtrN
-
Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
S-adenosyl-L-methionine pH 8.0, 28┬░C Niallia circulans
0.033
-
2-deoxy-scyllo-inosamine pH 8.0, 28┬░C Niallia circulans

General Information

General Information Comment Organism
physiological function gene disruption causes the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine and 2-deoxystreptamine. In vitro assay of the overexpressed enzyme reveals that the enzyme catalyzes the oxidation of 2-deoxy-scyllo-inosamine under strictly anaerobic conditions along with consumption of an equimolar amount of S-adenosyl-L-methionine to produce 5-deoxyadenosine, methionine, and 3-amino-2,3-dideoxy-scyllo-inosose Niallia circulans