BRENDA - Enzyme Database
show all sequences of 1.1.99.38

Mechanistic study on the reaction of a radical SAM dehydrogenase BtrN by electron paramagnetic resonance spectroscopy

Yokoyama, K.; Ohmori, D.; Kudo, F.; Eguchi, T.; Biochemistry 47, 8950-8960 (2008)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
analysis of electron paramagnetic resonance signals for free enzyme, the enzyme-S-adenosyl-L-methionine complex, and a rhombic signal presumably derived from the enzyme-S-adenosyl-L-methionine-substrate ternary complex show close interaction of both S-adenosyl-L-methione and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster
Bacillus circulans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.31
-
S-adenosyl-L-methionine
pH 8.0, 28°C, EDTA-treated enzyme
Bacillus circulans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
close interaction of both S-adenosyl-L-methionine and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster
Bacillus circulans
Organism
Organism
UniProt
Commentary
Textmining
Bacillus circulans
Q8G907
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine
radical S-adenosyl-L-methionine enzyme. Ketone formation is the rate-determining step in this reaction. Reaction involves formation of a 2-deoxy-scyllo-inosamine radical which can easily be converted to a highly reductive ketyl radical by deprotonation through proton-coupled electron transfer
685280
Bacillus circulans
3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
BtrN
-
Bacillus circulans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.021
-
S-adenosyl-L-methionine
pH 8.0, 28°C, EDTA-treated enzyme
Bacillus circulans
Cofactor
Cofactor
Commentary
Organism
Structure
Fe-S center
one [4Fe-4S] cluster per monomer, close interaction of both S-adenosyl-L-methione and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster
Bacillus circulans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Fe-S center
one [4Fe-4S] cluster per monomer, close interaction of both S-adenosyl-L-methione and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster
Bacillus circulans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
analysis of electron paramagnetic resonance signals for free enzyme, the enzyme-S-adenosyl-L-methionine complex, and a rhombic signal presumably derived from the enzyme-S-adenosyl-L-methionine-substrate ternary complex show close interaction of both S-adenosyl-L-methione and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster
Bacillus circulans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.31
-
S-adenosyl-L-methionine
pH 8.0, 28°C, EDTA-treated enzyme
Bacillus circulans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
close interaction of both S-adenosyl-L-methionine and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster
Bacillus circulans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine
radical S-adenosyl-L-methionine enzyme. Ketone formation is the rate-determining step in this reaction. Reaction involves formation of a 2-deoxy-scyllo-inosamine radical which can easily be converted to a highly reductive ketyl radical by deprotonation through proton-coupled electron transfer
685280
Bacillus circulans
3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.021
-
S-adenosyl-L-methionine
pH 8.0, 28°C, EDTA-treated enzyme
Bacillus circulans
Other publictions for EC 1.1.99.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740576
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137
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Bacillus circulans
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718869
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A consensus mechanism for radi ...
Bacillus circulans
Biochemistry
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2010
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685280
Yokoyama
Mechanistic study on the react ...
Bacillus circulans
Biochemistry
47
8950-8960
2008
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687282
Yokoyama
Characterization and mechanist ...
Bacillus circulans
J. Am. Chem. Soc.
129
15147-15155
2007
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