Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity | Amycolatopsis methanolica | |
additional information | no activation by phosphate and ammonium chloride. NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity | Amycolatopsis methanolica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-butanol | substrate inhibition | Amycolatopsis methanolica | |
1-propanol | substrate inhibition | Amycolatopsis methanolica | |
2-butanol | substrate inhibition | Amycolatopsis methanolica | |
2-propanol | substrate inhibition | Amycolatopsis methanolica | |
acetaldehyde | 1 mM, 40% inhibition; 40% inhibition at 1 mM, 60% at 2 mM, and 95% at 10 mM | Amycolatopsis methanolica | |
ADP | 10% inhibition at 1 mM, 50% at 10 mM | Amycolatopsis methanolica | |
AMP | 10% inhibition at 1 mM, 50% at 10 mM | Amycolatopsis methanolica | |
ATP | 10% inhibition at 1 mM, 50% at 10 mM | Amycolatopsis methanolica | |
Cu2+ | 1 mM, 90% inhibition; 90% inhibition at 1 mM | Amycolatopsis methanolica | |
ethanol | substrate inhibition | Amycolatopsis methanolica | |
Fe2+ | 1 mM, 90% inhibition; 90% inhibition at 1 mM | Amycolatopsis methanolica | |
Hg2+ | 100% inhibition at 1 mM | Amycolatopsis methanolica | |
KCN | 2 mM, 90% inhibition; 90% inhibition at 2 mM | Amycolatopsis methanolica | |
additional information | NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity; NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity | Amycolatopsis methanolica | |
NaN3 | 25% inhibition at 2 mM; 2 mM, 25% inhibition | Amycolatopsis methanolica | |
Ni2+ | 15% inhibition at 1 mM | Amycolatopsis methanolica | |
Zn2+ | 1 mM, 40% inhibition; 40% inhibition at 1 mM | Amycolatopsis methanolica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Amycolatopsis methanolica | |
0.0025 | - |
1-butanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
0.0039 | - |
1-propanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
0.082 | - |
ethanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
10.1 | - |
2-butanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
13.4 | - |
2-propanol | pH 7.0, 45°C | Amycolatopsis methanolica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
3 * 39000, SDS-PAGE | Amycolatopsis methanolica |
110000 | - |
PAGE | Amycolatopsis methanolica |
110000 | - |
native PAGE | Amycolatopsis methanolica |
120000 | - |
gel filtration | Amycolatopsis methanolica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + N,N-dimethyl-4-nitrosoaniline | Amycolatopsis methanolica | - |
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
ethanol + N,N-dimethyl-4-nitrosoaniline | Amycolatopsis methanolica NCIB 11946 | - |
acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
methanol + N,N-dimethyl-4-nitrosoaniline | Amycolatopsis methanolica | - |
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
methanol + N,N-dimethyl-4-nitrosoaniline | Amycolatopsis methanolica NCIB 11946 | - |
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis methanolica | - |
- |
- |
Amycolatopsis methanolica | P80175 | - |
- |
Amycolatopsis methanolica NCIB 11946 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 163fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, anion exchange and hydroxyapatite chromatography, followed by gel filtration | Amycolatopsis methanolica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.003 | - |
substrate methanol, pH 7.0, 45°C, extracts of cells grown on ethanol | Amycolatopsis methanolica |
0.004 | - |
substrate ethanol, pH 7.0, 45°C, extracts of cells grown on ethanol | Amycolatopsis methanolica |
0.005 | - |
substrate methanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol | Amycolatopsis methanolica |
0.018 | - |
substrate ethanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol | Amycolatopsis methanolica |
3.1 | - |
45°C, pH 7.0, purified enzyme | Amycolatopsis methanolica |
3.1 | - |
purified enzyme, pH 7.0, 45°C | Amycolatopsis methanolica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | butanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
1-butanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | butanal + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
1-butanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica NCIB 11946 | butanal + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
1-hexanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | hexanal + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
1-propanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | propanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
2-butanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | butanone + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
2-propanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | acetone + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
acetate + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | ? + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
ethanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
ethanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica NCIB 11946 | acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
methanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica | formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
methanol + N,N-dimethyl-4-nitrosoaniline | best substrate | Amycolatopsis methanolica | formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
methanol + N,N-dimethyl-4-nitrosoaniline | - |
Amycolatopsis methanolica NCIB 11946 | formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
methanol + N,N-dimethyl-4-nitrosoaniline | best substrate | Amycolatopsis methanolica NCIB 11946 | formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
? | |
additional information | enzyme shows formaldehyde dismutase activity producing methylformate when incubated with formaldehyde and methanol | Amycolatopsis methanolica | ? | - |
? | |
additional information | no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c | Amycolatopsis methanolica | ? | - |
? | |
additional information | no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c | Amycolatopsis methanolica NCIB 11946 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 39000, SDS-PAGE | Amycolatopsis methanolica |
Synonyms | Comment | Organism |
---|---|---|
4-nitroso-N, N-dimethylaniline-dependent alcohol dehydrogenase | - |
Amycolatopsis methanolica |
NDMA-ADH | - |
Amycolatopsis methanolica |
NDMA-dependent alcohol dehydrogenase | - |
Amycolatopsis methanolica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Amycolatopsis methanolica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Amycolatopsis methanolica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADPH | Amycolatopsis methanolica | |
NADH | 1 mol NADH per mol of enzyme trimer | Amycolatopsis methanolica | |
NADH | tightly bound, but NDMA-dependent alcohol dehydrogenase is unable to use NAD(P)(H) as cofactors | Amycolatopsis methanolica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
1-butanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
4 | - |
1-propanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
4.4 | - |
2-butanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
14.5 | - |
2-propanol | pH 7.0, 45°C | Amycolatopsis methanolica | |
33 | - |
ethanol | pH 7.0, 45°C | Amycolatopsis methanolica |