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Literature summary for 1.1.99.35 extracted from

  • Matsushita, K.; Toyama, H.; Ameyama, M.; Adachi, O.; Dewanti, A.; Duine, J.A.
    Soluble and membrane-bound quinoprotein D-glucose dehydrogenases of the Acinetobacter calcoaceticus: the binding process of PQQ to the apoenzymes (1995), Biosci. Biotechnol. Biochem., 59, 1548-1555.
No PubMed abstract available
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Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ or Cd2+, Sr2+, or Mn2+, required for binding of cofactor PQQ in soluble isoform sGDH, 100% activity with Ca2+. Mg2+, or Ca2+, Zn2+, or Sr2+, required for binding of cofactor PQQ in membrane-bound isoform mGDH. 100% activity with Ca2+ Acinetobacter calcoaceticus
Cd2+ or Ca2+, Sr2+, or Mn2+, required for binding of cofactor PQQ in soluble isoform sGDH. Mg2+, or Ca2+, Zn2+, or Sr2+, required for binding of cofactor PQQ in membrane-bound isoform mGDH Acinetobacter calcoaceticus
Mg2+ or Ca2+, Zn2+, or Sr2+, required for binding of cofactor PQQ in membrane-bound isoform mGDH. With Mg2+, 115% of the activity with Ca2+ Acinetobacter calcoaceticus
Mn2+ or Ca2+, Sr2+, or Cd2+, required for binding of cofactor PQQ in soluble isoform sGDH. Mg2+, or Ca2+, Zn2+, or Sr2+, required for binding of cofactor PQQ in membrane-bound isoform mGDH Acinetobacter calcoaceticus
Sr2+ or Ca2+, Cd2+, or Mn2+, required for binding of cofactor PQQ in soluble isoform sGDH, with Sr2+, 67% of the activity with Ca2+. Mg2+, or Ca2+, Zn2+, or Sr2+, required for binding of cofactor PQQ in membrane-bound isoform mGDH, with Sr2+, 70% of the activity with Ca2+ Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Synonyms

Synonyms Comment Organism
mGDH
-
 Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone soluble isoform sGDH is able to bind two mol of PQQ in one mol of the homodimer with high affinity. The binding reaction is much faster at alkaline pH than at acidic pH and requires the presence of some divalent cations such as Cd2+, Ca2+, Sr2+, or Mn2+. Membrane-bound isoform mGDH binds one mol of PQQ in the monomeric enzyme with a relatively slow reaction process, which has an optimim at acidic pH and in the presence of divalent cations such as Mg2+, Ca2+, Zn2+, Sr2+. Binding of PQQ affects the conformation of both isoforms Acinetobacter calcoaceticus