Application | Comment | Organism |
---|---|---|
synthesis | expression of enzyme in Pichia pastoris using the Saccharomyces cerevisiae alpha-factor signal sequence for secretion. The productivity of secreted PQQGDH-B achieves 218 kU/liter, i.e. 43 mg/liter. The secreted PQQGDH-B in Pichia pastoris is glycosylated but shows similar enzymatic properties as compared with those of recombinant PQQGDH-B produced in Escherichia coli | Acinetobacter calcoaceticus |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Acinetobacter calcoaceticus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression in Pichia pastoris using the Saccharomyces cerevisiae alpha-factor signal sequence for secretion. The productivity of secreted PQQGDH-B achieves 218 kU/liter, i.e. 43 mg/liter. The secreted PQQGDH-B in Pichia pastoris is glycosylated but shows similar enzymatic properties as compared with those of recombinant PQQGDH-B produced in Escherichia coli | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.5 | - |
D-glucose | recombinant enzyme, expression in Pichia pastoris | Acinetobacter calcoaceticus | |
17.5 | - |
lactose | recombinant enzyme, expression in Pichia pastoris | Acinetobacter calcoaceticus | |
18.9 | - |
lactose | recombinant enzyme, expression in Escherichia coli | Acinetobacter calcoaceticus | |
25 | - |
D-glucose | recombinant enzyme, expression in Escherichia coli | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the secreted PQQGDH-B in Pichia pastoris is glycosylated but shows similar enzymatic properties as compared with those of recombinant PQQGDH-B produced in Escherichia coli | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + oxidized 2,6-dichlorophenol-indophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
lactose + oxidized 2,6-dichlorophenol-indophenol | - |
Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenol-indophenol | - |
? |