Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.99.31 extracted from

  • Dewanti, A.R.; Mitra, B.
    A transient intermediate in the reaction catalyzed by (S)-mandelate dehydrogenase from Pseudomonas putida (2003), Biochemistry, 42, 12893-12901.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
(S)-Mandelate pH 7.5, 4°C Pseudomonas putida
0.12
-
(S)-Mandelate pH 7.5, 20°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelate + acceptor acceptor: 2,6-dichloroindophenol plus phenazine methosulfate. The MDH reaction has two rate-limiting step of similar activation energies: the formation and breakdown of a distinct intermediate, with the latter step being slightly more rate limiting. MDH is capable of catalyzing the reverse reaction, the reoxidation of reduced MDH by the product ketoacid, benzoylformate. The transient intermediate is observed during the reverse reaction as well Pseudomonas putida 2-oxo-2-phenylacetate + reduced acceptor
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
134
-
(S)-Mandelate pH 7.5, 4°C Pseudomonas putida
360
-
(S)-Mandelate pH 7.5, 20°C Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FMN
-
Pseudomonas putida