BRENDA - Enzyme Database show
show all sequences of 1.1.99.3

Overexpression of membrane-bound gluconate-2-dehydrogenase to enhance the production of 2-keto-D-gluconic acid by Gluconobacter oxydans

Li, K.; Mao, X.; Liu, L.; Lin, J.; Sun, M.; Wei, D.; Yang, S.; Microb. Cell Fact. 15, 121 (2016)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Gluconobacter oxydans
16020
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans DSM 2003
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-gluconate + acceptor
-
740963
Gluconobacter oxydans
2-dehydro-D-gluconate + reduced acceptor
-
-
-
?
D-gluconate + acceptor
-
740963
Gluconobacter oxydans DSM 2003
2-dehydro-D-gluconate + reduced acceptor
-
-
-
?
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Gluconobacter oxydans
16020
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-gluconate + acceptor
-
740963
Gluconobacter oxydans
2-dehydro-D-gluconate + reduced acceptor
-
-
-
?
D-gluconate + acceptor
-
740963
Gluconobacter oxydans DSM 2003
2-dehydro-D-gluconate + reduced acceptor
-
-
-
?
Other publictions for EC 1.1.99.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740963
Li
Overexpression of membrane-bou ...
Gluconobacter oxydans, Gluconobacter oxydans DSM 2003
Microb. Cell Fact.
15
121
2016
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695781
Saichana
Screening of thermotolerant Gl ...
Gluconobacter sp.
Appl. Environ. Microbiol.
75
4240-4247
2009
-
-
1
-
-
-
-
-
1
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
697598
Tsujimura
-
Direct electron transfer react ...
Gluconobacter frateurii, Gluconobacter frateurii NBRC 3271
Electrochemistry
76
549-551
2008
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
700265
Pajaniappan
A temperature-regulated Campyl ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Mol. Microbiol.
68
474-491
2008
-
-
1
-
2
-
-
-
2
-
2
4
-
2
-
-
-
-
-
-
6
-
4
1
1
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-
-
1
-
-
1
-
2
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-
-
1
1
-
2
-
-
-
-
-
2
-
2
4
-
-
-
-
-
-
6
-
4
1
1
-
-
-
1
-
-
2
1
-
-
1
-
-
684567
Toyama
Membrane-bound, 2-keto-D-gluco ...
Gluconobacter oxydans
Appl. Environ. Microbiol.
73
6551-6556
2007
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
-
-
-
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-
-
1
-
-
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-
-
1
1
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-
-
-
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-
1
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-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654319
Elfari
A Gluconobacter oxydans mutant ...
Gluconobacter oxydans
Appl. Microbiol. Biotechnol.
66
668-674
2005
-
-
-
-
-
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-
-
1
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1
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1
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1
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1
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1
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1
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-
-
-
-
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-
-
-
-
656696
Banta
Mathematical modeling of in vi ...
Pantoea agglomerans
Metab. Eng.
4
273-284
2002
-
-
-
-
-
-
-
-
1
-
-
1
-
1
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1
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1
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1
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-
1
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-
389920
Yum
Cloning and expression of a ge ...
Pantoea cypripedii
J. Bacteriol.
179
6566-6572
1997
-
-
1
-
-
-
-
-
-
-
3
-
-
1
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-
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1
1
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1
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3
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1
1
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-
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-
33649
Stowers
-
Gluconate catabolism in cowpea ...
Rhizobium sp.
Arch. Microbiol.
137
3-9
1984
-
-
-
-
-
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1
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1
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1
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1
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1
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-
389918
Shinagawa
-
D-Gluconate dehydrogenase, 2-k ...
Gluconobacter oxydans
Agric. Biol. Chem.
48
1517-1522
1984
-
-
-
-
-
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-
1
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3
-
-
1
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1
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1
-
2
1
1
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-
-
1
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2
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2
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1
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3
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1
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1
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2
1
1
-
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-
1
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-
-
389917
Matsushita
D-Gluconate dehydrogenase from ...
Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens
Methods Enzymol.
89
187-193
1982
-
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8
5
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6
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5
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2
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2
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7
4
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-
-
3
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3
3
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3
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8
3
5
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-
6
-
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2
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2
-
7
4
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-
3
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-
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-
-
389916
Matsushita
Membrane-bound D-gluconate deh ...
Pseudomonas aeruginosa
J. Biochem.
85
1173-1181
1979
1
-
-
-
-
-
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1
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5
-
-
2
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1
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1
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1
2
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2
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1
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2
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1
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5
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1
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1
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1
2
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-
389919
Matsushita
Membrane-bound D-gluconate deh ...
Pseudomonas aeruginosa
J. Biochem.
86
249-256
1979
-
-
-
-
-
-
6
6
1
-
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-
2
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5
-
1
-
1
-
3
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-
7
-
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-
-
-
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-
6
7
6
1
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5
-
1
-
1
-
3
-
-
-
-
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-
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-
389915
Shinagawa
-
Membrane-bound D-gluconate deh ...
Serratia marcescens
Agric. Biol. Chem.
42
2355-2361
1978
-
-
-
-
-
-
4
1
1
-
4
-
-
1
-
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1
-
-
-
1
-
1
1
1
-
3
-
1
1
1
1
-
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1
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4
-
1
1
-
4
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1
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1
-
1
1
1
-
3
-
1
1
1
-
-
-
-
-
-
-
389914
Ramakrishnan
Gluconic dehydrogenase of Pseu ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
17
122-127
1955
-
-
-
-
-
1
-
-
-
-
-
-
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2
-
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1
-
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-
1
2
1
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1
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1
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1
2
1
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