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Literature summary for 1.1.99.3 extracted from
- D-Gluconate dehydrogenase from bacteria, 2-keto-D-gluconate-yielding, membrane-bound (1982), Methods Enzymol., 89, 187-193.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oxalate | - |
Klebsiella pneumoniae |  |
| oxalate | - |
Pseudomonas aeruginosa | |
| oxamate | - |
Klebsiella pneumoniae | |
| oxamate | - |
Pseudomonas aeruginosa | |
| oxamate | - |
Serratia marcescens | |
| pyruvate | - |
Klebsiella pneumoniae | |
| pyruvate | - |
Pseudomonas aeruginosa | |
| pyruvate | - |
Serratia marcescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
D-gluconate | pH 6 | Pseudomonas fluorescens | |
| 0.8 | - |
D-gluconate | - |
Serratia marcescens | |
| 0.8 | - |
D-gluconate | pH 6 | Pseudomonas aeruginosa | |
| 0.8 | - |
D-gluconate | pH 6 | Klebsiella pneumoniae | |
| 2.9 | - |
D-gluconate | pH 4 | Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21700 | - |
1 * 21700 + 1 * 47500 + 1 * 66500, SDS-PAGE | Pseudomonas fluorescens |
| 22000 | - |
1 * 22000 + 1 * 52000 + 1 * 66000, SDS-PAGE | Klebsiella pneumoniae |
| 47500 | - |
1 * 21700 + 1 * 47500 + 1 * 66500, SDS-PAGE | Pseudomonas fluorescens |
| 52000 | - |
1 * 22000 + 1 * 52000 + 1 * 66000, SDS-PAGE | Klebsiella pneumoniae |
| 66000 | - |
1 * 22000 + 1 * 52000 + 1 * 66000, SDS-PAGE | Klebsiella pneumoniae |
| 66500 | - |
1 * 21700 + 1 * 47500 + 1 * 66500, SDS-PAGE | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
| Serratia marcescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
- |
Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 470 | - |
- |
Pseudomonas fluorescens |
| 513 | - |
- |
Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gluconate + 2,6-dichlorophenolindophenol | - |
Pseudomonas fluorescens | 2-oxogluconate + ? | - |
? | |
| D-gluconate + 2,6-dichlorophenolindophenol | - |
Pseudomonas aeruginosa | 2-oxogluconate + ? | - |
? | |
| D-gluconate + 2,6-dichlorophenolindophenol | - |
Serratia marcescens | 2-oxogluconate + ? | - |
? | |
| D-gluconate + 2,6-dichlorophenolindophenol | - |
Klebsiella pneumoniae | 2-oxogluconate + ? | - |
? | |
| D-gluconate + ferricyanide | - |
Pseudomonas fluorescens | 2-oxogluconate + ferrocyanide | - |
? | |
| D-gluconate + ferricyanide | - |
Pseudomonas aeruginosa | 2-oxogluconate + ferrocyanide | - |
? | |
| D-gluconate + ferricyanide | - |
Klebsiella pneumoniae | 2-oxogluconate + ferrocyanide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Pseudomonas aeruginosa |
| trimer | - |
Serratia marcescens |
| trimer | 1 * 22000 + 1 * 52000 + 1 * 66000, SDS-PAGE | Klebsiella pneumoniae |
| trimer | 1 * 21700 + 1 * 47500 + 1 * 66500, SDS-PAGE | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
reaction with ferricyanide | Klebsiella pneumoniae |
| 5 | - |
- |
Serratia marcescens |
| 5 | - |
reaction with ferricyanide | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 8alpha-(N1-histidyl)-FAD | prosthetic group | Pseudomonas fluorescens | |
| heme | contains 16.8 nmol of heme per mg of protein | Klebsiella pneumoniae | |
| heme | contains 14.6 nmol of heme per mg of protein | Pseudomonas fluorescens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
oxamate | - |
Klebsiella pneumoniae | |
| 2.8 | - |
oxalate | - |
Klebsiella pneumoniae | |
| 18.3 | - |
pyruvate | - |
Klebsiella pneumoniae | |
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