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Literature summary for 1.1.99.3 extracted from
- Membrane-bound D-gluconate dehydrogenase from Pseudomonas aeruginosa. Purification and structure of cytochrome-binding form (1979), J. Biochem., 85, 1173-1181.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | stimulates in presence of Triton X-100 | Pseudomonas aeruginosa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Pseudomonas aeruginosa | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE | Pseudomonas aeruginosa |
| 50000 | - |
1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE | Pseudomonas aeruginosa |
| 66000 | - |
1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE | Pseudomonas aeruginosa |
| 124000 | 131000 | sucrose density gradient centrifugation | Pseudomonas aeruginosa |
| 132000 | 138000 | non-denaturing PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 561.5 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gluconate + 2,6-dichlorophenolindophenol | - |
Pseudomonas aeruginosa | 2-oxogluconate + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | in absence of Triton X-100 the enzyme becomes dimeric | Pseudomonas aeruginosa |
| trimer | 1 * 22000, subunit of unclear function, + 1 * 50000, cytochrome c1, + 1 * 66000, flavoprotein, SDS-PAGE | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c1 | contains a cytochrome c1, c-554(551), which might by a diheme cytochrome | Pseudomonas aeruginosa | |
| flavin | contains covalently bound flavin | Pseudomonas aeruginosa | |
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