Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain CBS 7435 | Leucoagaricus meleagris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
ferrocenium ion | native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.16 | - |
ferrocenium ion | recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.49 | - |
L-arabinose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.54 | - |
L-arabinose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.55 | - |
2-chloro-1,4-benzoquinone | native enzyme, at pH 5.0 and 30°C | Leucoagaricus meleagris | |
0.62 | - |
2-chloro-1,4-benzoquinone | recombinant enzyme, at pH 5.0 and 30°C | Leucoagaricus meleagris | |
0.69 | - |
D-glucose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.82 | - |
D-glucose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
1.05 | - |
D-galactose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
1.07 | - |
D-galactose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
1.38 | - |
1,4-benzoquinone | recombinant enzyme, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
1.82 | - |
1,4-benzoquinone | native enzyme, at pH 3.0 and 30°C | Leucoagaricus meleagris | |
1.92 | - |
D-xylose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
1.93 | - |
D-xylose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
128 | - |
lactose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
134 | - |
lactose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61967 | - |
x * 61967, calculated from amino acid sequence | Leucoagaricus meleagris |
64000 | - |
x * 64000, deglycosylated protein, SDS-PAGE | Leucoagaricus meleagris |
93000 | - |
x * 93000, recombinant enzyme, SDS-PAGE | Leucoagaricus meleagris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + ferrocenium ion | Leucoagaricus meleagris | - |
2-dehydro-D-galactose + ferrocene | - |
? | |
D-glucose + 1,4-benzoquinone | Leucoagaricus meleagris | - |
? | - |
? | |
D-glucose + 2-chloro-1,4-benzoquinone | Leucoagaricus meleagris | - |
? | - |
? | |
D-glucose + ferrocenium ion | Leucoagaricus meleagris | - |
3-dehydro-D-glucose + ferrocene | - |
? | |
D-xylose + ferrocenium ion | Leucoagaricus meleagris | - |
? + ferrocene | - |
? | |
L-arabinose + ferrocenium ion | Leucoagaricus meleagris | - |
? + ferrocene | - |
? | |
lactose + ferrocenium ion | Leucoagaricus meleagris | - |
? + ferrocene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucoagaricus meleagris | Q3L245 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the level of glycosylation of recombinant enzyme is approximately 30% | Leucoagaricus meleagris |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography and phenyl Sepharose column chromatography | Leucoagaricus meleagris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.5 | - |
culture supernatant, at pH 8.5 and 30°C | Leucoagaricus meleagris |
21.9 | - |
after 1.9fold purification, at pH 8.5 and 30°C | Leucoagaricus meleagris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + ferrocenium ion | - |
Leucoagaricus meleagris | 2-dehydro-D-galactose + ferrocene | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Leucoagaricus meleagris | ? | - |
? | |
D-glucose + 2-chloro-1,4-benzoquinone | - |
Leucoagaricus meleagris | ? | - |
? | |
D-glucose + ferrocenium ion | - |
Leucoagaricus meleagris | 3-dehydro-D-glucose + ferrocene | - |
? | |
D-xylose + ferrocenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
L-arabinose + ferrocenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
lactose + ferrocenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61967, calculated from amino acid sequence | Leucoagaricus meleagris |
? | x * 64000, deglycosylated protein, SDS-PAGE | Leucoagaricus meleagris |
? | x * 93000, recombinant enzyme, SDS-PAGE | Leucoagaricus meleagris |
Synonyms | Comment | Organism |
---|---|---|
PDH1 | - |
Leucoagaricus meleagris |
pyranose dehydrogenase | - |
Leucoagaricus meleagris |
pyranose/acceptor oxidoreductase | - |
Leucoagaricus meleagris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.9 | - |
2-chloro-1,4-benzoquinone | recombinant enzyme, at pH 5.0 and 30°C | Leucoagaricus meleagris | |
15.1 | - |
2-chloro-1,4-benzoquinone | native enzyme, at pH 5.0 and 30°C | Leucoagaricus meleagris | |
34.7 | - |
L-arabinose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
37.2 | - |
L-arabinose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
37.8 | - |
D-glucose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
39.6 | - |
lactose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
41 | - |
lactose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
43.4 | - |
D-xylose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
45.9 | - |
D-glucose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
47.3 | - |
D-galactose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
48.5 | - |
D-galactose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
62.4 | - |
D-xylose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
65.4 | - |
1,4-benzoquinone | recombinant enzyme, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
76 | - |
1,4-benzoquinone | native enzyme, at pH 3.0 and 30°C | Leucoagaricus meleagris | |
104 | - |
ferrocenium ion | native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
130 | - |
ferrocenium ion | recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Leucoagaricus meleagris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
lactose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
0.32 | - |
lactose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
20.9 | - |
2-chloro-1,4-benzoquinone | recombinant enzyme, at pH 5.0 and 30°C | Leucoagaricus meleagris | |
22.9 | - |
D-xylose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
25.5 | - |
2-chloro-1,4-benzoquinone | native enzyme, at pH 5.0 and 30°C | Leucoagaricus meleagris | |
32.5 | - |
D-xylose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
44.2 | - |
D-galactose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
46.2 | - |
D-galactose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
54.4 | - |
1,4-benzoquinone | recombinant enzyme, at pH 4.0 and 30°C | Leucoagaricus meleagris | |
54.8 | - |
D-glucose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
57.5 | - |
1,4-benzoquinone | native enzyme, at pH 3.0 and 30°C | Leucoagaricus meleagris | |
57.5 | - |
D-glucose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
62.1 | - |
L-arabinose | using ferrocenium ion as cosubstrate, native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
70.8 | - |
L-arabinose | using ferrocenium ion as cosubstrate, recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
802 | - |
ferrocenium ion | native enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris | |
812 | - |
ferrocenium ion | recombinant enzyme, at pH 8.5 and 30°C | Leucoagaricus meleagris |