Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.99.29 extracted from

  • Volc, J.; Kubátová, E.; Daniel, G.; Sedmera, P.; Haltrich, D.
    Screening of basidiomycete fungi for the quinone-dependent sugar C-2/C-3 oxidoreductase, pyranose dehydrogenase, and properties of the enzyme from Macrolepiota rhacodes (2001), Arch. Microbiol., 176, 178-186.
    View publication on PubMed
Search for more literature for 1.1.99.29

General Stability

General Stability Organism
repeated freezing and thawing, two cycles, in the absence of glycerol has no effect on PDH activity Chlorophyllum rhacodes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.82
-
 D-glucose 25°C, ferricenium ion as electron acceptor Chlorophyllum rhacodes
24
-
 L-arabinose 25°C, ferricenium ion as electron acceptor Chlorophyllum rhacodes
29.2
-
 D-xylose 25°C, ferricenium ion as electron acceptor Chlorophyllum rhacodes
29.8
-
 D-galactose 25°C, ferricenium ion as electron acceptor Chlorophyllum rhacodes

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular active extracellular secretion of PDH Chlorophyllum rhacodes
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 gel filtration Chlorophyllum rhacodes
78000
-
 1 * 78000, SDS-PAGE Chlorophyllum rhacodes

Organism

Organism UniProt Comment Textmining
Chlorophyllum rhacodes
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein heavily glycosylated, the sugar moiety of PDH comprises 17.3% of its total molecular mass Chlorophyllum rhacodes

Purification (Commentary)

Purification (Comment) Organism
46fold Chlorophyllum rhacodes

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Chlorophyllum rhacodes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
67
-
 25°C, D-glucose and ferricenium ion as substrates Chlorophyllum rhacodes

Storage Stability

Storage Stability Organism
4°C, purified PDH, 20 mM sodium phosphate buffer, pH 7, 0.02% ProClin 300, 2 months, no loss of activity Chlorophyllum rhacodes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + 1,4-benzoquinone 49% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
2-deoxy-D-glucose + ferricenium ion 21% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
3-dehydro-D-glucose + 1,4-benzoquinone
-
 Chlorophyllum rhacodes 2,3-didehydro-D-glucose + hydroquinone
-
 ?
a pyranoside + acceptor
-
 Chlorophyllum rhacodes a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
-
 ?
cellobiose + 1,4-benzoquinone 13% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
cellobiose + ferricenium ion 7% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-galactose + 1,4-benzoquinone 66% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-galactose + ferricenium ion 60% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-glucono-1,5-lactone + 1,4-benzoquinone 20% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-glucono-1,5-lactone + ferricenium ion 8% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-glucose + 1,4-benzoquinone oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate Chlorophyllum rhacodes 3-dehydro-D-glucose + hydroquinone
-
 ?
D-glucose + ferricenium ion oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate Chlorophyllum rhacodes 3-dehydro-D-glucose + ferrocene
-
 ?
D-lyxose + 1,4-benzoquinone 5% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-lyxose + ferricenium ion 5% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-mannose + 1,4-benzoquinone 32% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-mannose + ferricenium ion 26% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-xylose + 1,4-benzoquinone 72% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
D-xylose + ferricenium ion 81% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
L-arabinose + 1,4-benzoquinone 54% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
L-arabinose + ferricenium ion 72% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
L-glucose + ferricenium ion 11% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
maltose + 1,4-benzoquinone 64% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
maltose + ferricenium ion 45% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
maltotriose + 1,4-benzoquinone 21% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
maltotriose + ferricenium ion 14% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone 11% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
methyl-alpha-D-galactopyranoside + ferricenium ion 8% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone 141% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
methyl-alpha-D-glucopyranoside + ferricenium ion 143% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone 30% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
methyl-beta-D-glucopyranoside + ferricenium ion 17% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
additional information broad selectivity for sugar substrates, O2 does not act as oxidant, not: D-allose, D-altrose, D-arabinose, D-ribose, D-fructose, L-sorbose, lactose, D-arabinitol, dulcitol, D-mannitol Chlorophyllum rhacodes ?
-
 ?
pyranose + acceptor
-
 Chlorophyllum rhacodes 2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
-
 ?
sucrose + 1,4-benzoquinone 9% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
sucrose + ferricenium ion 6% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
trehalose + 1,4-benzoquinone 7% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
trehalose + ferricenium ion 8% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 78000, SDS-PAGE Chlorophyllum rhacodes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 ferricenium ion as electron acceptor Chlorophyllum rhacodes
75
-
 1,4-benzoquinone as electron acceptor Chlorophyllum rhacodes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 50 mM sodium citrate buffer, 1,4-benzoquinone as electron acceptor Chlorophyllum rhacodes
9.5
-
 50 mM glycine buffer, ferricenium ion as electron acceptor Chlorophyllum rhacodes

Cofactor

Cofactor Comment Organism Structure
FAD functions as the cofactor Chlorophyllum rhacodes
flavin flavoprotein, FAD as cofactor Chlorophyllum rhacodes

pI Value

Organism Comment pI Value Maximum pI Value
Chlorophyllum rhacodes isoelectric focusing
-
 4.2