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Literature summary for 1.1.99.28 extracted from

  • Zhang, X.; Chen, G.; Liu, W.
    Reduction of xylose to xylitol catalyzed by glucose-fructose oxidoreductase from Zymomonas mobilis (2009), FEMS Microbiol. Lett., 293, 214-219.
    View publication on PubMed
Search for more literature for 1.1.99.28

Cloned(Commentary)

Cloned (Comment) Organism
His-tag Zymomonas mobilis

Protein Variants

Protein Variants Comment Organism
S116D dual NADP+/NAD+ specificity with loss of tight cofactor binding, wild type: strict NADP+ specificity Zymomonas mobilis
Y269F essential acid-base catalyst, involved in substrate binding, activity completely abolished Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis Q07982 ATCC 31821
-

Purification (Commentary)

Purification (Comment) Organism
-
 Zymomonas mobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.102
-
 for D-xylitol Zymomonas mobilis
68.39
-
 for D-sorbitol Zymomonas mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + D-fructose
-
 Zymomonas mobilis D-glucono-1,5-lactone + D-sorbitol
-
 ?
D-glucose + D-xylose
-
 Zymomonas mobilis D-gluconolactone + D-xylitol
-
 ?

Synonyms

Synonyms Comment Organism
glucose-fructose oxidoreductase
-
 Zymomonas mobilis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Zymomonas mobilis