Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | treatment of the membrane fraction causes a clear decrease in D-pentonate 4-dehydrogenase activity, activity is reactivated to almost the original level by the addition of pyrroloquinoline quinone and Ca2+ | Gluconobacter thailandicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.7 | - |
D-ribonate | pH 5.0, 25°C | Gluconobacter thailandicus | |
10.5 | - |
D-arabonate | pH 5.0, 25°C | Gluconobacter thailandicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gluconobacter thailandicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | EDTA-treated enzyme is reactivated to almost the original level by the addition of pyrroloquinoline quinone and Ca2+ | Gluconobacter thailandicus | |
additional information | metallic ions, Mg2+, Sr2+, Ba2+, and several kinds of lantanides, are ineffective in reactivation of EDTA-trreated enzyme | Gluconobacter thailandicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter thailandicus | M9MJR9 | - |
- |
Gluconobacter thailandicus NBRC 3255 | M9MJR9 | - |
- |
Purification (Comment) | Organism |
---|---|
n-octyl-beta-D-thioglucoside, n-octyl-beta-D-glucoside, Mydol 10, and Mydol 12 are effective detergents to solubilize the enzyme | Gluconobacter thailandicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | D-xylulose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-arabonate + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | 4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-arabonate + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus NBRC 3255 | 4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-erythronate + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | 3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-erythronate + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus NBRC 3255 | 3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-fructose + oxidized 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | 5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-fructose + oxidized 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus NBRC 3255 | 5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-gluconate + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | 5-dehydro-D-gluconate + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-psicose + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | 5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-psicose + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus NBRC 3255 | 5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-ribitol + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-ribitol + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus NBRC 3255 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-ribonate + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | 4-dehydro-D-ribonate + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-sorbitol + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | L-sorbose + reduced 2,6-dichlorophenolindophenol | - |
? | |
glycerol + 2,6-dichlorophenolindophenol | - |
Gluconobacter thailandicus | dihydroxyacetone + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x *14000, plus x * 80000, SDS-PAGE | Gluconobacter thailandicus |
Synonyms | Comment | Organism |
---|---|---|
D-sorbitol dehydrogenase subunit SldA | - |
Gluconobacter thailandicus |
GLDH | - |
Gluconobacter thailandicus |
NBRC3255_0235 | - |
Gluconobacter thailandicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Gluconobacter thailandicus |