BRENDA - Enzyme Database show
show all sequences of 1.1.99.22

Functionalization of the cytochrome P450cam monooxygenase system in the cell-like aqueous compartments of water-in-oil emulsions

Michizoe, J.; Ichinose, H.; Kamiya, N.; Maruyama, T.; Goto, M.; J. Biosci. Bioeng. 99, 12-17 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli BL21(DE3)pLysS as His-tag fusion protein
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli JM109
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity by anion exchange chromatography and Ni-affinity chromatography
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerol + NAD+
-
674959
Escherichia coli
dihydroxyacetone + NADH
-
-
-
?
glycerol + NAD+
-
674959
Escherichia coli JM109
dihydroxyacetone + NADH
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL21(DE3)pLysS as His-tag fusion protein
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity by anion exchange chromatography and Ni-affinity chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerol + NAD+
-
674959
Escherichia coli
dihydroxyacetone + NADH
-
-
-
?
glycerol + NAD+
-
674959
Escherichia coli JM109
dihydroxyacetone + NADH
-
-
-
?
Other publictions for EC 1.1.99.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740188
Ano
Membrane-bound glycerol dehydr ...
Gluconobacter thailandicus, Gluconobacter thailandicus NBRC 3258
Biosci. Biotechnol. Biochem.
81
411-418
2017
-
-
-
-
-
-
1
-
1
2
1
-
-
3
-
-
1
-
-
-
-
-
16
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
2
1
-
-
-
-
1
-
-
-
-
16
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721386
Hattori
High-temperature sorbose ferme ...
Gluconobacter frateurii, Gluconobacter frateurii CHM43, NBRC 101659
Appl. Microbiol. Biotechnol.
95
1531-1540
2012
-
-
1
-
-
-
-
-
-
1
-
2
-
2
-
-
-
-
-
1
-
-
6
-
1
1
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
6
-
1
1
-
-
1
-
-
-
-
1
1
-
-
-
721866
Habe
Disruption of the membrane-bou ...
Gluconobacter oxydans, Gluconobacter oxydans NBRC12528
Biosci. Biotechnol. Biochem.
74
1391-1395
2010
-
-
-
-
-
-
-
-
2
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721867
Habe
Use of a Gluconobacter frateur ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
74
2330-2332
2010
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695781
Saichana
Screening of thermotolerant Gl ...
Gluconobacter sp., Gluconobacter sp. THE42
Appl. Environ. Microbiol.
75
4240-4247
2009
-
-
-
-
-
-
-
-
-
1
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685690
Soemphol
Distinct physiological roles o ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
72
842-850
2008
-
-
-
-
-
-
-
-
1
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668061
Ichinose
Enzymatic redox cofactor regen ...
Escherichia coli, Escherichia coli JM109
Biotechnol. Prog.
21
1192-1197
2005
-
-
1
-
-
-
-
-
-
1
-
-
-
31
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
674959
Michizoe
Functionalization of the cytoc ...
Escherichia coli, Escherichia coli JM109
J. Biosci. Bioeng.
99
12-17
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
31
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654267
Matsushita
5-Keto-D-gluconate production ...
Gluconobacter oxydans
Appl. Environ. Microbiol.
69
1959-1966
2003
-
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287791
Ameyama
-
Solubilization, purification a ...
Gluconobacter oxydans, Gluconobacter oxydans IFO 3260
Agric. Biol. Chem.
49
1001-1010
1985
-
-
-
-
-
3
1
4
1
-
-
-
-
2
-
-
1
-
-
-
1
2
13
-
1
-
-
-
1
-
1
4
-
-
-
-
-
-
4
-
-
3
-
1
-
4
1
-
-
-
-
-
-
1
-
-
1
2
13
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-