BRENDA - Enzyme Database show
show all sequences of 1.1.99.22

Solubilization, purification and properties of membrane-bound glycerol dehydrogenase from Gluconobacter industrius

Ameyama, M.; Shinagawa, E.; Matsushita, K.; Adachi, O.; Agric. Biol. Chem. 49, 1001-1010 (1985)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
amphoteric detergents, such as dimethyldodecylamineoxide, lauroylsarcosine, dodecylsulfobetaine, lauroylbetaine are effective solubilizers
Gluconobacter oxydans
dimethyldodecylamineoxide, 0.5%, stabilizes
Gluconobacter oxydans
glycerol stabilizes
Gluconobacter oxydans
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3
-
glycerol
membrane fraction
Gluconobacter oxydans
19
-
meso-erythritol
-
Gluconobacter oxydans
22
-
D-arabitol
-
Gluconobacter oxydans
34
-
glycerol
solubilized enzyme
Gluconobacter oxydans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Gluconobacter oxydans
16020
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
-
maximum activity at the end of exponential growth phase, activity occurs in various Gluconobacter species, such as Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter suboxydans and Gluconobacter albidus
-
Gluconobacter oxydans IFO 3260
-
maximum activity at the end of exponential growth phase, activity occurs in various Gluconobacter species, such as Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter suboxydans and Gluconobacter albidus
-
Purification (Commentary)
Commentary
Organism
-
Gluconobacter oxydans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
52
-
-
Gluconobacter oxydans
Storage Stability
Storage Stability
Organism
-20C, solubilized, stable for 6 months
Gluconobacter oxydans
2-4C, diluted enzyme solution, stable for 2 weeks
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adonitol + acceptor
42% of activity with glycerol
287791
Gluconobacter oxydans
?
-
-
-
?
adonitol + acceptor
42% of activity with glycerol
287791
Gluconobacter oxydans IFO 3260
?
-
-
-
?
D-arabitol + acceptor
136% of activity with glycerol oxidation
287791
Gluconobacter oxydans
?
-
-
-
?
D-arabitol + acceptor
136% of activity with glycerol oxidation
287791
Gluconobacter oxydans IFO 3260
?
-
-
-
?
D-mannitol + acceptor
26% of activity with glycerol
287791
Gluconobacter oxydans
D-fructose + reduced acceptor
-
287791
Gluconobacter oxydans
?
D-sorbitol + acceptor
45% of acticvity with glycerol
287791
Gluconobacter oxydans
L-sorbose + reduced acceptor
-
287791
Gluconobacter oxydans
?
D-sorbitol + acceptor
45% of acticvity with glycerol
287791
Gluconobacter oxydans IFO 3260
L-sorbose + reduced acceptor
-
287791
Gluconobacter oxydans IFO 3260
?
galactitol + acceptor
i.e. dulcitol, 29% of activity with glycerol
287791
Gluconobacter oxydans
?
-
-
-
?
glycerol + acceptor
2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde
287791
Gluconobacter oxydans
glycerone + reduced acceptor
i.e. dihydroxyacetone
287791
Gluconobacter oxydans
?
glycerol + acceptor
2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde
287791
Gluconobacter oxydans IFO 3260
glycerone + reduced acceptor
i.e. dihydroxyacetone
287791
Gluconobacter oxydans IFO 3260
?
meso-erythritol + acceptor
111% of activity with glycerol
287791
Gluconobacter oxydans
?
-
-
-
?
meso-erythritol + acceptor
111% of activity with glycerol
287791
Gluconobacter oxydans IFO 3260
?
-
-
-
?
propylene glycol + acceptor
54% of activity with glycerol
287791
Gluconobacter oxydans
?
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Gluconobacter oxydans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8.5
10
stable in the presence of glycerol and detergent
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
acts in vitro as acceptor together with phenazine methosulfate
Gluconobacter oxydans
additional information
NAD+, NADP+ or molecular oxygen do not act as acceptors
Gluconobacter oxydans
phenazine methosulfate
acts in vitro as acceptor together with 2,6-dichlorophenolindophenol, linked to electron-transport chain of the organism
Gluconobacter oxydans
pyrroloquinoline quinone
quinoprotein
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
acts in vitro as acceptor together with phenazine methosulfate
Gluconobacter oxydans
additional information
NAD+, NADP+ or molecular oxygen do not act as acceptors
Gluconobacter oxydans
phenazine methosulfate
acts in vitro as acceptor together with 2,6-dichlorophenolindophenol, linked to electron-transport chain of the organism
Gluconobacter oxydans
pyrroloquinoline quinone
quinoprotein
Gluconobacter oxydans
General Stability (protein specific)
General Stability
Organism
amphoteric detergents, such as dimethyldodecylamineoxide, lauroylsarcosine, dodecylsulfobetaine, lauroylbetaine are effective solubilizers
Gluconobacter oxydans
dimethyldodecylamineoxide, 0.5%, stabilizes
Gluconobacter oxydans
glycerol stabilizes
Gluconobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3
-
glycerol
membrane fraction
Gluconobacter oxydans
19
-
meso-erythritol
-
Gluconobacter oxydans
22
-
D-arabitol
-
Gluconobacter oxydans
34
-
glycerol
solubilized enzyme
Gluconobacter oxydans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Gluconobacter oxydans
16020
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gluconobacter oxydans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
52
-
-
Gluconobacter oxydans
Storage Stability (protein specific)
Storage Stability
Organism
-20C, solubilized, stable for 6 months
Gluconobacter oxydans
2-4C, diluted enzyme solution, stable for 2 weeks
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adonitol + acceptor
42% of activity with glycerol
287791
Gluconobacter oxydans
?
-
-
-
?
adonitol + acceptor
42% of activity with glycerol
287791
Gluconobacter oxydans IFO 3260
?
-
-
-
?
D-arabitol + acceptor
136% of activity with glycerol oxidation
287791
Gluconobacter oxydans
?
-
-
-
?
D-arabitol + acceptor
136% of activity with glycerol oxidation
287791
Gluconobacter oxydans IFO 3260
?
-
-
-
?
D-mannitol + acceptor
26% of activity with glycerol
287791
Gluconobacter oxydans
D-fructose + reduced acceptor
-
287791
Gluconobacter oxydans
?
D-sorbitol + acceptor
45% of acticvity with glycerol
287791
Gluconobacter oxydans
L-sorbose + reduced acceptor
-
287791
Gluconobacter oxydans
?
D-sorbitol + acceptor
45% of acticvity with glycerol
287791
Gluconobacter oxydans IFO 3260
L-sorbose + reduced acceptor
-
287791
Gluconobacter oxydans IFO 3260
?
galactitol + acceptor
i.e. dulcitol, 29% of activity with glycerol
287791
Gluconobacter oxydans
?
-
-
-
?
glycerol + acceptor
2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde
287791
Gluconobacter oxydans
glycerone + reduced acceptor
i.e. dihydroxyacetone
287791
Gluconobacter oxydans
?
glycerol + acceptor
2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde
287791
Gluconobacter oxydans IFO 3260
glycerone + reduced acceptor
i.e. dihydroxyacetone
287791
Gluconobacter oxydans IFO 3260
?
meso-erythritol + acceptor
111% of activity with glycerol
287791
Gluconobacter oxydans
?
-
-
-
?
meso-erythritol + acceptor
111% of activity with glycerol
287791
Gluconobacter oxydans IFO 3260
?
-
-
-
?
propylene glycol + acceptor
54% of activity with glycerol
287791
Gluconobacter oxydans
?
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Gluconobacter oxydans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8.5
10
stable in the presence of glycerol and detergent
Gluconobacter oxydans
Other publictions for EC 1.1.99.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740188
Ano
Membrane-bound glycerol dehydr ...
Gluconobacter thailandicus, Gluconobacter thailandicus NBRC 3258
Biosci. Biotechnol. Biochem.
81
411-418
2017
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-
-
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-
1
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1
2
1
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3
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1
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16
1
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1
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1
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1
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1
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1
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1
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16
1
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721386
Hattori
High-temperature sorbose ferme ...
Gluconobacter frateurii, Gluconobacter frateurii CHM43, NBRC 101659
Appl. Microbiol. Biotechnol.
95
1531-1540
2012
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1
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1
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2
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2
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1
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6
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1
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1
2
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1
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6
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1
1
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1
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1
1
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-
721866
Habe
Disruption of the membrane-bou ...
Gluconobacter oxydans, Gluconobacter oxydans NBRC12528
Biosci. Biotechnol. Biochem.
74
1391-1395
2010
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2
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4
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2
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4
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4
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1
1
-
-
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721867
Habe
Use of a Gluconobacter frateur ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
74
2330-2332
2010
-
-
1
-
1
-
-
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5
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1
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1
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695781
Saichana
Screening of thermotolerant Gl ...
Gluconobacter sp., Gluconobacter sp. THE42
Appl. Environ. Microbiol.
75
4240-4247
2009
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1
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2
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4
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2
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1
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1
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1
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2
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685690
Soemphol
Distinct physiological roles o ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
72
842-850
2008
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1
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2
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7
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4
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1
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2
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4
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668061
Ichinose
Enzymatic redox cofactor regen ...
Escherichia coli, Escherichia coli JM109
Biotechnol. Prog.
21
1192-1197
2005
-
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1
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1
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31
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1
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1
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1
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1
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1
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2
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2
1
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674959
Michizoe
Functionalization of the cytoc ...
Escherichia coli, Escherichia coli JM109
J. Biosci. Bioeng.
99
12-17
2005
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1
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31
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654267
Matsushita
5-Keto-D-gluconate production ...
Gluconobacter oxydans
Appl. Environ. Microbiol.
69
1959-1966
2003
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1
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1
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1
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1
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8
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1
1
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1
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1
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8
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1
1
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287791
Ameyama
-
Solubilization, purification a ...
Gluconobacter oxydans, Gluconobacter oxydans IFO 3260
Agric. Biol. Chem.
49
1001-1010
1985
-
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3
1
4
1
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2
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1
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1
2
13
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1
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1
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4
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4
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3
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1
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4
1
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1
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1
2
13
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1
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1
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1
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