BRENDA - Enzyme Database
show all sequences of 1.1.99.22

Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-dehydro-D-pentonates, D-fructose to 5-dehydro-D-fructose, and D-psicose to 5-dehydro-D-psicose

Ano, Y.; Hours, R.A.; Akakabe, Y.; Kataoka, N.; Yakushi, T.; Matsushita, K.; Adachi, O.; Biosci. Biotechnol. Biochem. 81, 411-418 (2017)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Gluconobacter thailandicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Gluconobacter thailandicus
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates
Gluconobacter thailandicus
additional information
Mg2+, Sr2+, Ba2+, and several kinds of lantanides do not activate the enzyme
Gluconobacter thailandicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
x * 80000, SDS-PAGE
Gluconobacter thailandicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter thailandicus
-
-
-
Gluconobacter thailandicus NBRC 3258
-
-
-
Purification (Commentary)
Commentary
Organism
CM-Toyopearl C-650 column chromatography, and Sephadex G-200 gel filtration
Gluconobacter thailandicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-arabitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-arabonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-arabonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-erythronate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-erythronate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-fructose + oxidized 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-fructose + oxidized 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-psicose + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-psicose + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-ribitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-ribonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
4-dehydro-D-ribonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-sorbitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glycerol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
glycerone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glycerol + phenazine methosulfate
-
740188
Gluconobacter thailandicus
glycerone + reduced phenazine methosulfate
-
-
-
?
glycerol + potassium ferricyanide
-
740188
Gluconobacter thailandicus
glycerone + potassium ferrocyanide
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 80000, SDS-PAGE
Gluconobacter thailandicus
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Gluconobacter thailandicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Gluconobacter thailandicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Gluconobacter thailandicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Gluconobacter thailandicus
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates
Gluconobacter thailandicus
additional information
Mg2+, Sr2+, Ba2+, and several kinds of lantanides do not activate the enzyme
Gluconobacter thailandicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
x * 80000, SDS-PAGE
Gluconobacter thailandicus
Purification (Commentary) (protein specific)
Commentary
Organism
CM-Toyopearl C-650 column chromatography, and Sephadex G-200 gel filtration
Gluconobacter thailandicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-arabitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-arabonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-arabonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-erythronate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-erythronate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-fructose + oxidized 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-fructose + oxidized 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-psicose + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-psicose + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-ribitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-ribonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
4-dehydro-D-ribonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
D-sorbitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glycerol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
glycerone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glycerol + phenazine methosulfate
-
740188
Gluconobacter thailandicus
glycerone + reduced phenazine methosulfate
-
-
-
?
glycerol + potassium ferricyanide
-
740188
Gluconobacter thailandicus
glycerone + potassium ferrocyanide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 80000, SDS-PAGE
Gluconobacter thailandicus
Other publictions for EC 1.1.99.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740188
Ano
Membrane-bound glycerol dehydr ...
Gluconobacter thailandicus, Gluconobacter thailandicus NBRC 3258
Biosci. Biotechnol. Biochem.
81
411-418
2017
-
-
-
-
-
-
1
-
1
2
1
-
-
3
-
-
1
-
-
-
-
-
16
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
2
1
-
-
-
-
1
-
-
-
-
16
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721386
Hattori
High-temperature sorbose ferme ...
Gluconobacter frateurii, Gluconobacter frateurii CHM43, NBRC 101659
Appl. Microbiol. Biotechnol.
95
1531-1540
2012
-
-
1
-
-
-
-
-
-
1
-
2
-
2
-
-
-
-
-
1
-
-
6
-
1
1
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
6
-
1
1
-
-
1
-
-
-
-
1
1
-
-
-
721866
Habe
Disruption of the membrane-bou ...
Gluconobacter oxydans, Gluconobacter oxydans NBRC12528
Biosci. Biotechnol. Biochem.
74
1391-1395
2010
-
-
-
-
-
-
-
-
2
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721867
Habe
Use of a Gluconobacter frateur ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
74
2330-2332
2010
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695781
Saichana
Screening of thermotolerant Gl ...
Gluconobacter sp., Gluconobacter sp. THE42
Appl. Environ. Microbiol.
75
4240-4247
2009
-
-
-
-
-
-
-
-
-
1
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685690
Soemphol
Distinct physiological roles o ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
72
842-850
2008
-
-
-
-
-
-
-
-
1
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668061
Ichinose
Enzymatic redox cofactor regen ...
Escherichia coli, Escherichia coli JM109
Biotechnol. Prog.
21
1192-1197
2005
-
-
1
-
-
-
-
-
-
1
-
-
-
31
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
674959
Michizoe
Functionalization of the cytoc ...
Escherichia coli, Escherichia coli JM109
J. Biosci. Bioeng.
99
12-17
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
31
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654267
Matsushita
5-Keto-D-gluconate production ...
Gluconobacter oxydans
Appl. Environ. Microbiol.
69
1959-1966
2003
-
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287791
Ameyama
-
Solubilization, purification a ...
Gluconobacter oxydans, Gluconobacter oxydans IFO 3260
Agric. Biol. Chem.
49
1001-1010
1985
-
-
-
-
-
3
1
4
1
-
-
-
-
2
-
-
1
-
-
-
1
2
13
-
1
-
-
-
1
-
1
4
-
-
-
-
-
-
4
-
-
3
-
1
-
4
1
-
-
-
-
-
-
1
-
-
1
2
13
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-