BRENDA - Enzyme Database
show all sequences of 1.1.99.22

High-temperature sorbose fermentation with thermotolerant Gluconobacter frateurii CHM43 and its mutant strain adapted to higher temperature

Hattori, H.; Yakushi, T.; Matsutani, M.; Moonmangmee, D.; Toyama, H.; Adachi, O.; Matsushita, K.; Appl. Microbiol. Biotechnol. 95, 1531-1540 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene sldBA, DNA and amino acid sequence determination and analysis
Gluconobacter frateurii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Gluconobacter frateurii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
Gluconobacter frateurii
-
2-oxoglutarate + pyrroloquinoline quinol
-
-
?
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
Gluconobacter frateurii CHM43, NBRC 101659
-
2-oxoglutarate + pyrroloquinoline quinol
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Gluconobacter frateurii
-
gene sldBA
-
Gluconobacter frateurii CHM43, NBRC 101659
-
gene sldBA
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
temperature increase reduces the enzyme activity, which can be restored by pyroloquinoline quinone. Adaptation of the CHM43 strain at 38.5C
Gluconobacter frateurii
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
-
721386
Gluconobacter frateurii
2-oxoglutarate + pyrroloquinoline quinol
-
-
-
?
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
coupling with 2,6-dichlorophenolindophenol
721386
Gluconobacter frateurii
2-oxoglutarate + pyrroloquinoline quinol
-
-
-
?
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
-
721386
Gluconobacter frateurii CHM43, NBRC 101659
2-oxoglutarate + pyrroloquinoline quinol
-
-
-
?
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
coupling with 2,6-dichlorophenolindophenol
721386
Gluconobacter frateurii CHM43, NBRC 101659
2-oxoglutarate + pyrroloquinoline quinol
-
-
-
?
additional information
the enzyme shows ubiquinol-2 oxidase activity
721386
Gluconobacter frateurii
?
-
-
-
-
additional information
the enzyme shows ubiquinol-2 oxidase activity
721386
Gluconobacter frateurii CHM43, NBRC 101659
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
PQQ-dependent GLDH
-
Gluconobacter frateurii
pyrroloquinoline quinone-dependent glycerol dehydrogenase
-
Gluconobacter frateurii
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Gluconobacter frateurii
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
additional information
-
temperature increase reduces the enzyme activity, which can be restores by pyroloquinoline quinone. Adaptation of the CHM43 strain at 38.5C
Gluconobacter frateurii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Gluconobacter frateurii
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the enzyme is actvie with 2,6-dichlorophenolindophenol
Gluconobacter frateurii
pyrroloquinoline quinone
-
Gluconobacter frateurii
Cloned(Commentary) (protein specific)
Commentary
Organism
gene sldBA, DNA and amino acid sequence determination and analysis
Gluconobacter frateurii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the enzyme is actvie with 2,6-dichlorophenolindophenol
Gluconobacter frateurii
pyrroloquinoline quinone
-
Gluconobacter frateurii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
required
Gluconobacter frateurii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
Gluconobacter frateurii
-
2-oxoglutarate + pyrroloquinoline quinol
-
-
?
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
Gluconobacter frateurii CHM43, NBRC 101659
-
2-oxoglutarate + pyrroloquinoline quinol
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
temperature increase reduces the enzyme activity, which can be restored by pyroloquinoline quinone. Adaptation of the CHM43 strain at 38.5C
Gluconobacter frateurii
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
-
721386
Gluconobacter frateurii
2-oxoglutarate + pyrroloquinoline quinol
-
-
-
?
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
coupling with 2,6-dichlorophenolindophenol
721386
Gluconobacter frateurii
2-oxoglutarate + pyrroloquinoline quinol
-
-
-
?
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
-
721386
Gluconobacter frateurii CHM43, NBRC 101659
2-oxoglutarate + pyrroloquinoline quinol
-
-
-
?
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
coupling with 2,6-dichlorophenolindophenol
721386
Gluconobacter frateurii CHM43, NBRC 101659
2-oxoglutarate + pyrroloquinoline quinol
-
-
-
?
additional information
the enzyme shows ubiquinol-2 oxidase activity
721386
Gluconobacter frateurii
?
-
-
-
-
additional information
the enzyme shows ubiquinol-2 oxidase activity
721386
Gluconobacter frateurii CHM43, NBRC 101659
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Gluconobacter frateurii
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
additional information
-
temperature increase reduces the enzyme activity, which can be restores by pyroloquinoline quinone. Adaptation of the CHM43 strain at 38.5C
Gluconobacter frateurii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Gluconobacter frateurii
General Information
General Information
Commentary
Organism
metabolism
pyrroloquinoline quinone-dependent glycerol dehydrogenase is a primary dehydrogenase of the respiratory chain and responsible for L-sorbose production
Gluconobacter frateurii
General Information (protein specific)
General Information
Commentary
Organism
metabolism
pyrroloquinoline quinone-dependent glycerol dehydrogenase is a primary dehydrogenase of the respiratory chain and responsible for L-sorbose production
Gluconobacter frateurii
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740188
Ano
Membrane-bound glycerol dehydr ...
Gluconobacter thailandicus, Gluconobacter thailandicus NBRC 3258
Biosci. Biotechnol. Biochem.
81
411-418
2017
-
-
-
-
-
-
1
-
1
2
1
-
-
3
-
-
1
-
-
-
-
-
16
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
2
1
-
-
-
-
1
-
-
-
-
16
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721386
Hattori
High-temperature sorbose ferme ...
Gluconobacter frateurii, Gluconobacter frateurii CHM43, NBRC 101659
Appl. Microbiol. Biotechnol.
95
1531-1540
2012
-
-
1
-
-
-
-
-
-
1
-
2
-
2
-
-
-
-
-
1
-
-
6
-
2
1
1
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
6
-
1
1
-
-
1
-
-
-
-
1
1
-
-
-
721866
Habe
Disruption of the membrane-bou ...
Gluconobacter oxydans, Gluconobacter oxydans NBRC12528
Biosci. Biotechnol. Biochem.
74
1391-1395
2010
-
-
-
-
-
-
-
-
2
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721867
Habe
Use of a Gluconobacter frateur ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
74
2330-2332
2010
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695781
Saichana
Screening of thermotolerant Gl ...
Gluconobacter sp., Gluconobacter sp. THE42
Appl. Environ. Microbiol.
75
4240-4247
2009
-
-
-
-
-
-
-
-
-
1
-
2
-
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685690
Soemphol
Distinct physiological roles o ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
72
842-850
2008
-
-
-
-
-
-
-
-
1
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668061
Ichinose
Enzymatic redox cofactor regen ...
Escherichia coli, Escherichia coli JM109
Biotechnol. Prog.
21
1192-1197
2005
-
-
1
-
-
-
-
-
-
1
-
-
-
31
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
674959
Michizoe
Functionalization of the cytoc ...
Escherichia coli, Escherichia coli JM109
J. Biosci. Bioeng.
99
12-17
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
31
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654267
Matsushita
5-Keto-D-gluconate production ...
Gluconobacter oxydans
Appl. Environ. Microbiol.
69
1959-1966
2003
-
-
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287791
Ameyama
-
Solubilization, purification a ...
Gluconobacter oxydans, Gluconobacter oxydans IFO 3260
Agric. Biol. Chem.
49
1001-1010
1985
-
-
-
-
-
3
1
4
1
-
-
-
-
2
-
-
1
-
-
-
1
2
13
-
1
1
-
-
-
1
-
1
4
-
-
-
-
-
-
4
-
-
3
-
1
-
4
1
-
-
-
-
-
-
1
-
-
1
2
13
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-