Application | Comment | Organism |
---|---|---|
synthesis | the most prominent industrial method of producing L-sorbose is the biotransformation of D-sorbitol to L-sorbose in Gluconobacter species or Acetobacter species. L-sorbose is an important carbohydrate that is predominantly used as a starting material in the biosynthesis of L-ascorbic acid | Gluconobacter oxydans |
Cloned (Comment) | Organism |
---|---|
gene sldhAB, enzyme overexpression in an industrial strain Gluconobacter oxydans WSH-003 with a strong promoter PtufB, subcloning in Escherichia coli strain JM109, quantitative real-time PCR expression analysis | Gluconobacter oxydans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | method evaluation and optimization for engineered L-sorbose production in Gluconobater oxydans by self-overexpressing the sldhAB gene in Gluconobacter oxydans strain WSH-003 with an optimal poly(A/T) tail under the constitutive promoter PtufB, the titer and the productivity of L-sorbose are enhanced by 36.3% and 25.0%, respectively, in a 1-L fermenter. Immobilization of Gluconobacter oxydans-sldhAB6 cells further improves the L-sorbose titer by 33.7% after 20 days of semi-continuous fed-batch fermentation. Immobilization of recombinant enzyme in calcium alginate beads, the L-sorbose titer is improved by 33.7% by the immobilization of sldhAB6 cells | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the membrane-bound dehydrogenase locates on the outer surface of the cytoplasmic membrane. The oxidation products accumulate in the culture medium | Gluconobacter oxydans | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol + acceptor | Gluconobacter oxydans | D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase | L-sorbose + reduced acceptor | the oxidation product accumulates in the culture medium | ? | |
D-sorbitol + acceptor | Gluconobacter oxydans WSH-003 | D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase | L-sorbose + reduced acceptor | the oxidation product accumulates in the culture medium | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q70JP0 AND Q70JN9 | small and large subunits, SldB and SldA | - |
Gluconobacter oxydans WSH-003 | Q70JP0 AND Q70JN9 | small and large subunits, SldB and SldA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol + acceptor | - |
Gluconobacter oxydans | L-sorbose + reduced acceptor | - |
? | |
D-sorbitol + acceptor | D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase | Gluconobacter oxydans | L-sorbose + reduced acceptor | the oxidation product accumulates in the culture medium | ? | |
D-sorbitol + acceptor | - |
Gluconobacter oxydans WSH-003 | L-sorbose + reduced acceptor | - |
? | |
D-sorbitol + acceptor | D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase | Gluconobacter oxydans WSH-003 | L-sorbose + reduced acceptor | the oxidation product accumulates in the culture medium | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
SLDH | - |
Gluconobacter oxydans |
sldhAB | - |
Gluconobacter oxydans |
sorbitol dehydrogenase | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Gluconobacter oxydans |