Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.99.21 extracted from

  • Xu, S.; Wang, X.; Du, G.; Zhou, J.; Chen, J.
    Enhanced production of L-sorbose from D-sorbitol by improving the mRNA abundance of sorbitol dehydrogenase in Gluconobacter oxydans WSH-003 (2014), Microb. Cell Fact., 13, 146.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the most prominent industrial method of producing L-sorbose is the biotransformation of D-sorbitol to L-sorbose in Gluconobacter species or Acetobacter species. L-sorbose is an important carbohydrate that is predominantly used as a starting material in the biosynthesis of L-ascorbic acid Gluconobacter oxydans

Cloned(Commentary)

Cloned (Comment) Organism
gene sldhAB, enzyme overexpression in an industrial strain Gluconobacter oxydans WSH-003 with a strong promoter PtufB, subcloning in Escherichia coli strain JM109, quantitative real-time PCR expression analysis Gluconobacter oxydans

Protein Variants

Protein Variants Comment Organism
additional information method evaluation and optimization for engineered L-sorbose production in Gluconobater oxydans by self-overexpressing the sldhAB gene in Gluconobacter oxydans strain WSH-003 with an optimal poly(A/T) tail under the constitutive promoter PtufB, the titer and the productivity of L-sorbose are enhanced by 36.3% and 25.0%, respectively, in a 1-L fermenter. Immobilization of Gluconobacter oxydans-sldhAB6 cells further improves the L-sorbose titer by 33.7% after 20 days of semi-continuous fed-batch fermentation. Immobilization of recombinant enzyme in calcium alginate beads, the L-sorbose titer is improved by 33.7% by the immobilization of sldhAB6 cells Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the membrane-bound dehydrogenase locates on the outer surface of the cytoplasmic membrane. The oxidation products accumulate in the culture medium Gluconobacter oxydans 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-sorbitol + acceptor Gluconobacter oxydans D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase L-sorbose + reduced acceptor the oxidation product accumulates in the culture medium ?
D-sorbitol + acceptor Gluconobacter oxydans WSH-003 D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase L-sorbose + reduced acceptor the oxidation product accumulates in the culture medium ?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans Q70JP0 AND Q70JN9 small and large subunits, SldB and SldA
-
Gluconobacter oxydans WSH-003 Q70JP0 AND Q70JN9 small and large subunits, SldB and SldA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol + acceptor
-
Gluconobacter oxydans L-sorbose + reduced acceptor
-
?
D-sorbitol + acceptor D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase Gluconobacter oxydans L-sorbose + reduced acceptor the oxidation product accumulates in the culture medium ?
D-sorbitol + acceptor
-
Gluconobacter oxydans WSH-003 L-sorbose + reduced acceptor
-
?
D-sorbitol + acceptor D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase Gluconobacter oxydans WSH-003 L-sorbose + reduced acceptor the oxidation product accumulates in the culture medium ?

Subunits

Subunits Comment Organism
dimer
-
Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
SLDH
-
Gluconobacter oxydans
sldhAB
-
Gluconobacter oxydans
sorbitol dehydrogenase
-
Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Gluconobacter oxydans