Literature summary for 1.1.99.2 extracted from

Huedig, M.; Maier, A.; Scherrers, I.; Seidel, L.; Jansen, E.E.; Mettler-Altmann, T.; Engqvist, M.K.; Maurino, V.G.
Plants possess a cyclic mitochondrial metabolic pathway similar to the mammalian metabolic repair mechanism involving malate dehydrogenase and l-2-hydroxyglutarate dehydrogenase (2015), Plant Cell Physiol., 56, 1820-1830.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.24	-	(S)-2-hydroxyglutarate	at pH 7.4 and 30°C	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Arabidopsis thaliana	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	x * 46000, SDS-PAGE	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA agarose column chromatography	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-2-hydroxyglutarate + 2,6-dichlorophenolindophenol	3.7% activity compared to (S)-2-hydroxyglutarate	Arabidopsis thaliana	2-oxoglutarate + reduced 2,6-dichlorophenolindophenol	-	?
(S)-2-hydroxyglutarate + 2,6-dichlorophenolindophenol	-	Arabidopsis thaliana	2-oxoglutarate + reduced 2,6-dichlorophenolindophenol	-	?
additional information	the enzyme is not active in the presence of NAD+, NADP+, cytochrome c or O2, as well as glycolate, D-lactate and L-lactate	Arabidopsis thaliana	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 46000, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
L-2HG dehydrogenase	-	Arabidopsis thaliana
L-2HGDH	-	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
613	-	(S)-2-hydroxyglutarate	at pH 7.4 and 30°C	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	-	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)