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Literature summary for 1.1.99.18 extracted from

  • Balaz, A.M.; Stevanovic, J.; Ostafe, R.; Blazic, M.; Ilic Durdic, K.; Fischer, R.; Prodanovic, R.
    Semi-rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide (2020), Mol. Divers., 24, 593-601 .
    View publication on PubMed
Search for more literature for 1.1.99.18

Application

Application Comment Organism
biofuel production the enzyme can be used for constructing biofuel cells Phanerodontia chrysosporium
diagnostics the enzyme can be used for constructing biosensors Phanerodontia chrysosporium
industry the enzyme can be used for bleaching cotton in textile industry Phanerodontia chrysosporium
medicine application in biomedicine as an antimicrobial and antibiofilm agent Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
M65F mutant with increased activity and stability in the presence of peroxide. 70% of residual activity after 6 h of incubation in 0.3 M hydrogen peroxide, compared to wild-type CDH that retained 40% of original activity Phanerodontia chrysosporium
M685Y mutant with increased activity and stability in the presence of peroxide. 90% of residual activity after 6 h of incubation in 0.3 M hydrogen peroxide, compared to wild-type CDH that retained 40% of original activity. 2.5 times increased kcat for lactose compared to wild-type enzyme. The mutant enzyme is a good candidate for applications in biofuel cells and biocatalysis for lactobionic acid production Phanerodontia chrysosporium
M738S mutant with increased activity and stability in the presence of peroxide. 80% of residual activity after 6 h of incubation in 0.3 M hydrogen peroxide, compared to wild-type CDH that retained 40% of original activity Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
 cellobiose 25°C, pH 4.5, mutant enzyme M685Y Phanerodontia chrysosporium
0.038
-
 cellobiose 25°C, pH 4.5, wild-type enzyme Phanerodontia chrysosporium
0.103
-
 cellobiose 25°C, pH 4.5, mutant enzyme M65F Phanerodontia chrysosporium
0.162
-
 cellobiose 25°C, pH 4.5, mutant enzyme M738S Phanerodontia chrysosporium
0.77
-
 lactose 25°C, pH 4.5, wild-type enzyme Phanerodontia chrysosporium
1.43
-
 lactose 25°C, pH 4.5, mutant enzyme M65F Phanerodontia chrysosporium
1.44
-
 lactose 25°C, pH 4.5, mutant enzyme M685Y Phanerodontia chrysosporium
1.53
-
 lactose 25°C, pH 4.5, mutant enzyme M738S Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q01738
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichloroindophenol
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
lactose + 2,6-dichloroindophenol
-
 Phanerodontia chrysosporium 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?

Synonyms

Synonyms Comment Organism
Cdh
-
 Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.81
-
 cellobiose 25°C, pH 4.5, wild-type enzyme Phanerodontia chrysosporium
0.97
-
 lactose 25°C, pH 4.5, mutant enzyme M738S Phanerodontia chrysosporium
1.55
-
 lactose 25°C, pH 4.5, wild-type enzyme Phanerodontia chrysosporium
1.58
-
 cellobiose 25°C, pH 4.5, mutant enzyme M738S Phanerodontia chrysosporium
2.62
-
 cellobiose 25°C, pH 4.5, mutant enzyme M685Y Phanerodontia chrysosporium
3.27
-
 cellobiose 25°C, pH 4.5, mutant enzyme M65F Phanerodontia chrysosporium
3.95
-
 lactose 25°C, pH 4.5, mutant enzyme M685Y Phanerodontia chrysosporium
4.64
-
 lactose 25°C, pH 4.5, mutant enzyme M65F Phanerodontia chrysosporium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.64
-
 lactose 25°C, pH 4.5, mutant enzyme M738S Phanerodontia chrysosporium
2
-
 lactose 25°C, pH 4.5, wild-type enzyme Phanerodontia chrysosporium
2.73
-
 lactose 25°C, pH 4.5, mutant enzyme M685Y Phanerodontia chrysosporium
3.24
-
 lactose 25°C, pH 4.5, mutant enzyme M65F Phanerodontia chrysosporium
9.76
-
 cellobiose 25°C, pH 4.5, mutant enzyme M738S Phanerodontia chrysosporium
21.5
-
 cellobiose 25°C, pH 4.5, wild-type enzyme Phanerodontia chrysosporium
31.8
-
 cellobiose 25°C, pH 4.5, mutant enzyme M65F Phanerodontia chrysosporium
209
-
 cellobiose 25°C, pH 4.5, mutant enzyme M685Y Phanerodontia chrysosporium