Literature summary for 1.1.99.18 extracted from

Sulej, J.; Jaszek, M.; Osinska-Jaroszuk, M.; Matuszewska, A.; Bancerz, R.; Janczarek, M.
Natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase (2021), Arch. Microbiol., 203, 4433-4448 .

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General Stability

General Stability	Organism
the presence of polysaccharide preparations enhances the stability of cellobiose dehydrogenase compared to the control value at 4°C. The highest stabilization effect is observed for the enzyme modified with Rh110EPS	Trametes sanguinea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	cellobiose	addition of polysaccharides modifies the Km-value, depending on incubation temperature and incubation time	Trametes sanguinea
additional information	-	lactose	addition of polysaccharides modifies the Km-value, depending on incubation temperature and incubation time	Trametes sanguinea
0.158	-	cellobiose	pH 4.5, 30°C, without polysaccharide	Trametes sanguinea
2.9	-	lactose	pH 4.5, 30°C, without polysaccharide	Trametes sanguinea

Organism

Organism	UniProt	Comment	Textmining
Trametes sanguinea	S5RVR8	i.e. Pycnoporus sanguineus (FCL199)	-

Purification (Commentary)

Purification (Comment)	Organism
-	Trametes sanguinea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellobiose + 2,6-dichloroindophenol	-	Trametes sanguinea	cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol	-	?
lactose + 2,6-dichloroindophenol	-	Trametes sanguinea	4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol	-	?

Synonyms

Synonyms	Comment	Organism
Cdh	-	Trametes sanguinea

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	cellobiose	addition of polysaccharides modifies the turnover numer, depending on incubation temperature and incubation time	Trametes sanguinea
additional information	-	lactose	addition of polysaccharides modifies the turnover numer, depending on incubation temperature and incubation time	Trametes sanguinea
11.73	-	cellobiose	pH 4.5, 30°C, without polysaccharide	Trametes sanguinea
14.3	-	lactose	pH 4.5, 30°C, without polysaccharide	Trametes sanguinea

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	-	Trametes sanguinea

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Release 2023.1 (January 2023)