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Literature summary for 1.1.99.18 extracted from
- Characterization of a cellobiose dehydrogenase from Humicola insolens (1998), Biochem. J., 330, 565-571 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
cellobiose | pH 7.5, 40°C | Humicola insolens |  |
| 0.012 | - |
ferricyanide | pH 7.5, 40°C | Humicola insolens | |
| 0.017 | - |
cellopentaose | pH 7.5, 40°C | Humicola insolens | |
| 0.019 | - |
cellotriose | pH 7.5, 40°C | Humicola insolens | |
| 0.021 | - |
cellotetraose | pH 7.5, 40°C | Humicola insolens | |
| 0.026 | - |
2,6-dichlorophenolindophenol | pH 7.5, 40°C | Humicola insolens | |
| 0.051 | - |
lactose | pH 7.5, 40°C | Humicola insolens | |
| 0.093 | - |
cytochrome c | pH 7.5, 40°C | Humicola insolens | |
| 0.132 | - |
benzoquinone | pH 7.5, 40°C | Humicola insolens | |
| 7.1 | - |
xylobiose | pH 7.5, 40°C | Humicola insolens | |
| 11 | - |
maltose | pH 7.5, 40°C | Humicola insolens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Humicola insolens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
gel filtration | Humicola insolens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Humicola insolens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glycoprotein with a total sugar content of 2% | Humicola insolens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4409 | - |
pH 7.5, 40°C | Humicola insolens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + 2,6-dichloroindophenol | - |
Humicola insolens | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
| cellopentaose + 2,6-dichlorophenolindophenol | - |
Humicola insolens | cellopentono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| cellotetraose + acceptor | - |
Humicola insolens | cellotetraono-1,5-lactone + reduced acceptor | - |
? | |
| cellotriose + 2,6-dichlorophenol indophenol | - |
Humicola insolens | cellotriono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
| lactose + cytochrome c | - |
Humicola insolens | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
| lactose + methylene blue | - |
Humicola insolens | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced methylene blue | - |
? | |
| maltose + benzoquinone | - |
Humicola insolens | maltono-1,5-lactone + benzoquinol | - |
? | |
| additional information | possible electron acceptors are 2,6-dichlorophenolindophenol, methylene blue, 3,5-di-t-butyl-1,2-benzoquinone, potassium ferricyanide, cytochrome c and molecular oxygen | Humicola insolens | ? | - |
? | |
| xylobiose + ferricyanide | - |
Humicola insolens | 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + ferrocyanide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 92000, SDS-PAGE | Humicola insolens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Humicola insolens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
maltose | pH 7.5, 40°C | Humicola insolens | |
| 3 | - |
xylobiose | pH 7.5, 40°C | Humicola insolens | |
| 12 | - |
cellotriose | pH 7.5, 40°C | Humicola insolens | |
| 12 | - |
cellotetraose | pH 7.5, 40°C | Humicola insolens | |
| 12 | - |
cellopentaose | pH 7.5, 40°C | Humicola insolens | |
| 14 | - |
cellobiose | pH 7.5, 40°C | Humicola insolens | |
| 14 | - |
lactose | pH 7.5, 40°C | Humicola insolens | |
| 14 | - |
ferricyanide | pH 7.5, 40°C | Humicola insolens | |
| 17 | - |
2,6-dichlorophenolindophenol | pH 7.5, 40°C | Humicola insolens | |
| 21 | - |
benzoquinone | pH 7.5, 40°C | Humicola insolens | |
| 27 | - |
cytochrome c | pH 7.5, 40°C | Humicola insolens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH-optima for the oxidation vary remarkably depending on the electron acceptor | Humicola insolens |
| 4 | - |
acceptor methylene blue | Humicola insolens |
| 7 | - |
acceptor 2,6-dichloroindophenol | Humicola insolens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b | - |
Humicola insolens | |
| FAD | - |
Humicola insolens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Humicola insolens | isoelectric focusing | - |
4 |
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