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Literature summary for 1.1.99.18 extracted from

  • Mulla, D.; Kracher, D.; Ludwig, R.; Nagy, G.; Grandits, M.; Holzer, W.; Saber, Y.; Gabra, N.; Viernstein, H.; Unger, F.M.
    Azido derivatives of cellobiose: oxidation at C1 with cellobiose dehydrogenase from Sclerotium rolfsii (2013), Carbohydr. Res., 382, 86-94.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.131
-
cellobiose at pH 4.5 and 30°C Athelia rolfsii
0.72
-
6'-azido-6'-deoxy-beta-D-glucopyranosyl-(1->4)-D-glucopyranose at pH 4.5 and 30°C Athelia rolfsii
1.7
-
beta-D-glucopyranosyl-(1->4)-2-azido-2-deoxy-D-glucopyranose at pH 4.5 and 30°C Athelia rolfsii
1.77
-
beta-D-glucopyranosyl-(1->4)-6-azido-6-deoxy-D-glucopyranose at pH 4.5 and 30°C Athelia rolfsii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + acceptor Athelia rolfsii
-
cellobiono-1,5-lactone + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Athelia rolfsii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6'-azido-6'-deoxy-beta-D-glucopyranosyl-(1->4)-D-glucopyranose + 2,6-dichlorophenolindophenol
-
Athelia rolfsii ?
-
?
beta-D-glucopyranosyl-(1->4)-2-azido-2-deoxy-D-glucopyranose + 2,6-dichlorophenolindophenol
-
Athelia rolfsii ?
-
?
beta-D-glucopyranosyl-(1->4)-6-azido-6-deoxy-D-glucopyranose + 2,6-dichlorophenolindophenol
-
Athelia rolfsii ?
-
?
cellobiose + acceptor
-
Athelia rolfsii cellobiono-1,5-lactone + reduced acceptor
-
?

Synonyms

Synonyms Comment Organism
Cdh
-
Athelia rolfsii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
beta-D-glucopyranosyl-(1->4)-2-azido-2-deoxy-D-glucopyranose at pH 4.5 and 30°C Athelia rolfsii
9.1
-
beta-D-glucopyranosyl-(1->4)-6-azido-6-deoxy-D-glucopyranose at pH 4.5 and 30°C Athelia rolfsii
17.7
-
6'-azido-6'-deoxy-beta-D-glucopyranosyl-(1->4)-D-glucopyranose at pH 4.5 and 30°C Athelia rolfsii
27
-
cellobiose at pH 4.5 and 30°C Athelia rolfsii

Cofactor

Cofactor Comment Organism Structure
FAD
-
Athelia rolfsii
heme b
-
Athelia rolfsii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.53
-
beta-D-glucopyranosyl-(1->4)-2-azido-2-deoxy-D-glucopyranose at pH 4.5 and 30°C Athelia rolfsii
5.14
-
beta-D-glucopyranosyl-(1->4)-6-azido-6-deoxy-D-glucopyranose at pH 4.5 and 30°C Athelia rolfsii
24.6
-
6'-azido-6'-deoxy-beta-D-glucopyranosyl-(1->4)-D-glucopyranose at pH 4.5 and 30°C Athelia rolfsii
206
-
cellobiose at pH 4.5 and 30°C Athelia rolfsii