Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris X-33 cells | Thermothelomyces myriococcoides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
cellobiose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
0.085 | - |
cellotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
0.09 | - |
lactose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
0.71 | - |
maltotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
6.75 | - |
maltose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
387 | - |
D-glucose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces myriococcoides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and phenyl Sepharose column chromatography | Thermothelomyces myriococcoides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
culture supernatant, in 100 mM sodium acetate buffer, pH 5.0, at 30°C | Thermothelomyces myriococcoides |
3.1 | - |
after 15.5fold purification, in 100 mM sodium acetate buffer, pH 5.0, at 30°C | Thermothelomyces myriococcoides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
cellotetraose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
D-glucose + 2,6-dichlorophenol indophenol | weak substrate | Thermothelomyces myriococcoides | D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
lactose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
maltose + oxidized 2,6-dichlorophenolindophenol | - |
Thermothelomyces myriococcoides | 4-O-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
maltotetraose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | ? + reduced 2,6-dichlorophenol indophenol | - |
? | |
maltotriose + 2,6-dichlorophenol indophenol | - |
Thermothelomyces myriococcoides | ? + reduced 2,6-dichlorophenol indophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Thermothelomyces myriococcoides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.315 | - |
maltose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
0.48 | - |
maltotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
2.7 | - |
cellotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
4.11 | - |
cellobiose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
4.68 | - |
lactose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
4.8 | - |
D-glucose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
D-glucose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
0.047 | - |
maltose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
0.676 | - |
maltotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
32.12 | - |
cellotetraose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
52 | - |
lactose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides | |
117.1 | - |
cellobiose | in 100 mM sodium acetate buffer, pH 4.0, at 30°C | Thermothelomyces myriococcoides |