Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X33 | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0518 | - |
cellobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.0814 | - |
cellotriose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.0902 | - |
cellotetraose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.1131 | - |
cellopentaose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.1224 | - |
cellohexaose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.2 | - |
lactose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.74 | - |
Galactobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
1.53 | - |
mannobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
2.81 | - |
xylobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
634 | - |
galactose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
726 | - |
glucose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
5051 | - |
maltose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
5051 | - |
xylose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
5051 | - |
mannose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
mature peptide, calculated from amino acid sequence | Neurospora crassa |
120000 | - |
SDS-PAGE | Neurospora crassa |
130000 | - |
gel filtration | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Neurospora crassa FGSC 2489 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant enzyme contains approximately 20.83% carbohydrate | Neurospora crassa |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenol indophenol | best substrate | Neurospora crassa | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
cellobiose + 2,6-dichlorophenol indophenol | best substrate | Neurospora crassa FGSC 2489 | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
cellohexaose + 2,6-dichlorophenol indophenol | - |
Neurospora crassa | cellohexono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
cellohexaose + 2,6-dichlorophenol indophenol | - |
Neurospora crassa FGSC 2489 | cellohexono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
cellopentaose + 2,6-dichlorophenol indophenol | - |
Neurospora crassa | cellopentono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
cellotetraose + 2,6-dichlorophenol indophenol | - |
Neurospora crassa | cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
cellotetraose + 2,6-dichlorophenol indophenol | - |
Neurospora crassa FGSC 2489 | cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
cellotriose + 2,6-dichlorophenol indophenol | - |
Neurospora crassa | cellotriono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
galactobiose + 2,6-dichlorophenol indophenol | good substrate | Neurospora crassa | ? + reduced 2,6-dichlorophenol indophenol | - |
? | |
galactose + 2,6-dichlorophenol indophenol | weak substrate | Neurospora crassa | ? + reduced 2,6-dichlorophenol indophenol | - |
? | |
glucose + 2,6-dichlorophenol indophenol | weak substrate | Neurospora crassa | ? + reduced 2,6-dichlorophenol indophenol | - |
? | |
lactose + 2,6-dichlorophenol indophenol | good substrate | Neurospora crassa | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
lactose + 2,6-dichlorophenol indophenol | good substrate | Neurospora crassa FGSC 2489 | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
maltose + 2,6-dichlorophenol indophenol | - |
Neurospora crassa | ? + reduced 2,6-dichlorophenol indophenol | - |
? | |
mannobiose + 2,6-dichlorophenol indophenol | good substrate | Neurospora crassa | ? + reduced 2,6-dichlorophenol indophenol | - |
? | |
mannose + 2,6-dichlorophenol indophenol | weak substrate | Neurospora crassa | ? + reduced 2,6-dichlorophenol indophenol | - |
? | |
xylobiose + 2,6-dichlorophenol indophenol | good substrate | Neurospora crassa | 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
xylobiose + 2,6-dichlorophenol indophenol | good substrate | Neurospora crassa FGSC 2489 | 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
xylose + 2,6-dichlorophenol indophenol | weak substrate | Neurospora crassa | ? + reduced 2,6-dichlorophenol indophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDH1 | - |
Neurospora crassa |
cellobiose dehydrogenase | - |
Neurospora crassa |
cellobiose:[acceptor] 1-oxidoreductase | - |
Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | - |
Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the half life for the recombinant enzyme at 60°C is 20 min | Neurospora crassa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
Galactobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
2.1 | - |
galactose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
2.1 | - |
mannobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
3.7 | - |
xylobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
4.6 | - |
xylose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
7.6 | - |
glucose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
10.2 | - |
mannose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
11.1 | - |
lactose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
20.5 | - |
maltose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
23.9 | - |
cellotetraose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
24 | - |
cellobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
24.7 | - |
cellohexaose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
24.78 | - |
cellotriose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
25.7 | - |
cellopentaose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6 | the CDH activity decreases very sharply below pH 4.5. The recombinant enzyme remains at 60.8% of the maximum CDH activity at pH 4.0, and at only 22.6% CDH activity at pH 3.5 | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Neurospora crassa | |
heme | - |
Neurospora crassa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Neurospora crassa | mature peptide, calculated from amino acid sequence | - |
6.7 |
Neurospora crassa | isoelectric focusing | - |
6.8 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00091 | - |
xylose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.002 | - |
mannose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.0033 | - |
galactose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.004 | - |
maltose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
0.01 | - |
glucose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
1.33 | - |
xylobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
1.39 | - |
mannobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
1.92 | - |
Galactobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
55.4 | - |
lactose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
202.1 | - |
cellohexaose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
227 | - |
cellopentaose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
265 | - |
cellotetraose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
305.6 | - |
cellotriose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa | |
461.4 | - |
cellobiose | recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C | Neurospora crassa |