Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | complete inhibition at 2 mM | Chlamydomonas reinhardtii | |
EDTA | 8% inhibition at 10 mM | Chlamydomonas reinhardtii | |
KCN | complete inhibition at 2 mM | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
glycolate | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C | Chlamydomonas reinhardtii | |
0.45 | - |
D-lactate | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-lactate + 2,6-dichlorophenolindophenol | 100% activity with D-lactate and 2,6-dichlorophenolindophenol | Chlamydomonas reinhardtii | ? | - |
? | |
D-lactate + phenazine methosulfate | 93% activity compared to 2,6-dichlorophenolindophenol | Chlamydomonas reinhardtii | ? | - |
? | |
glycerate 3-phosphate + 2,6-dichlorophenolindophenol | 2.9% activity compared to D-lactate | Chlamydomonas reinhardtii | ? | - |
? | |
glycolate + 2,6-dichlorophenolindophenol | 71.7% activity compared to D-lactate | Chlamydomonas reinhardtii | glyoxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-lactate + 2,6-dichlorophenolindophenol | 12.4% activity compared to D-lactate | Chlamydomonas reinhardtii | ? | - |
? | |
additional information | no activity with formate, malate, acetate, FAD, FMN, NAD+, NADP+, or cytochrome c | Chlamydomonas reinhardtii | ? | - |
? | |
succinate + 2,6-dichlorophenolindophenol | 8.5% activity compared to D-lactate | Chlamydomonas reinhardtii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlcDH | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Chlamydomonas reinhardtii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.93 | - |
glycolate | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C | Chlamydomonas reinhardtii | |
5.42 | - |
L-lactate | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C | Chlamydomonas reinhardtii | |
5.42 | - |
D-lactate | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Chlamydomonas reinhardtii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.92 | - |
glycolate | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C | Chlamydomonas reinhardtii | |
13 | - |
D-lactate | with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C | Chlamydomonas reinhardtii |